Curated Information
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Curated Information Page
PubMed Id: 22474382 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Brumbaugh J, et al. (2012) Phosphorylation regulates human OCT4. Proc Natl Acad Sci U S A 109, 7162-8 22474382
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T92-p - Oct4 (human) ▲
Orthologous residues
Oct4 (human): T92‑p, Oct4 iso2 (human): ‑, Oct4 (mouse): T87‑p, Oct4 iso2 (mouse): ‑
Characterization
 Methods used to characterize site in vivo:  mass spectrometry
 Relevant cell lines - cell types - tissues:  ES (stem)
 Cellular systems studied:  cell lines
 Species studied:  human

S93-p - Oct4 (human) ▲
Orthologous residues
Oct4 (human): S93‑p, Oct4 iso2 (human): ‑, Oct4 (mouse): L88‑p, Oct4 iso2 (mouse): ‑
Characterization
 Methods used to characterize site in vivo:  mass spectrometry
 Relevant cell lines - cell types - tissues:  ES (stem)
 Cellular systems studied:  cell lines
 Species studied:  human

S105-p - Oct4 (human) ▲
Orthologous residues
Oct4 (human): S105‑p, Oct4 iso2 (human): ‑, Oct4 (mouse): S100‑p, Oct4 iso2 (mouse): ‑
Characterization
 Methods used to characterize site in vivo:  mass spectrometry
 Relevant cell lines - cell types - tissues:  ES (stem)
 Cellular systems studied:  cell lines
 Species studied:  human

S107-p - Oct4 (human) ▲
Orthologous residues
Oct4 (human): S107‑p, Oct4 iso2 (human): ‑, Oct4 (mouse): S102‑p, Oct4 iso2 (mouse): ‑
Characterization
 Methods used to characterize site in vivo:  mass spectrometry
 Relevant cell lines - cell types - tissues:  ES (stem)
 Cellular systems studied:  cell lines
 Species studied:  human

S111-p - Oct4 (human) ▲
Orthologous residues
Oct4 (human): S111‑p, Oct4 iso2 (human): ‑, Oct4 (mouse): S106‑p, Oct4 iso2 (mouse): ‑
Characterization
 Methods used to characterize site in vivo:  mass spectrometry
 Relevant cell lines - cell types - tissues:  ES (stem)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ERK2 (human)

T116-p - Oct4 (human) ▲
Orthologous residues
Oct4 (human): T116‑p, Oct4 iso2 (human): ‑, Oct4 (mouse): A111‑p, Oct4 iso2 (mouse): ‑
Characterization
 Methods used to characterize site in vivo:  mass spectrometry
 Relevant cell lines - cell types - tissues:  ES (stem)
 Cellular systems studied:  cell lines
 Species studied:  human

T118-p - Oct4 (human) ▲
Orthologous residues
Oct4 (human): T118‑p, Oct4 iso2 (human): ‑, Oct4 (mouse): R113‑p, Oct4 iso2 (mouse): ‑
Characterization
 Methods used to characterize site in vivo:  mass spectrometry
 Relevant cell lines - cell types - tissues:  ES (stem)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ERK2 (human)

T235-p - Oct4 (human) ▲
Orthologous residues
Oct4 (human): T235‑p, Oct4 iso2 (human): T140‑p, Oct4 (mouse): T228‑p, Oct4 iso2 (mouse): T97‑p
Characterization
 Methods used to characterize site in vivo:  electrophoretic mobility shift, mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  293FT, ES (stem)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  transcription, inhibited
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
DNA Disrupts

S236-p - Oct4 (human) ▲
Orthologous residues
Oct4 (human): S236‑p, Oct4 iso2 (human): S141‑p, Oct4 (mouse): S229‑p, Oct4 iso2 (mouse): S98‑p
Characterization
 Methods used to characterize site in vivo:  electrophoretic mobility shift, mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  293FT, ES (stem)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  transcription, inhibited
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
DNA Disrupts

S288-p - Oct4 (human) ▲
Orthologous residues
Oct4 (human): S288‑p, Oct4 iso2 (human): S193‑p, Oct4 (mouse): S281‑p, Oct4 iso2 (mouse): S150‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry
 Relevant cell lines - cell types - tissues:  ES (stem)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE PKACa (human)
KINASE Pim1 (human)

S289-p - Oct4 (human) ▲
Orthologous residues
Oct4 (human): S289‑p, Oct4 iso2 (human): S194‑p, Oct4 (mouse): S282‑p, Oct4 iso2 (mouse): S151‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry
 Relevant cell lines - cell types - tissues:  ES (stem)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE PKACa (human)
KINASE Pim1 (human)

T352-p - Oct4 (human) ▲
Orthologous residues
Oct4 (human): T352‑p, Oct4 iso2 (human): T257‑p, Oct4 (mouse): A344‑p, Oct4 iso2 (mouse): A213‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry
 Relevant cell lines - cell types - tissues:  ES (stem)
 Cellular systems studied:  cell lines
 Species studied:  human

S355-p - Oct4 (human) ▲
Orthologous residues
Oct4 (human): S355‑p, Oct4 iso2 (human): S260‑p, Oct4 (mouse): S347‑p, Oct4 iso2 (mouse): S216‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry
 Relevant cell lines - cell types - tissues:  ES (stem)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ERK2 (human)

S359-p - Oct4 (human) ▲
Orthologous residues
Oct4 (human): S359‑p, Oct4 iso2 (human): S264‑p, Oct4 (mouse): S351‑p, Oct4 iso2 (mouse): S220‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry
 Relevant cell lines - cell types - tissues:  ES (stem)
 Cellular systems studied:  cell lines
 Species studied:  human

Y125-p - SOX2 (human) ▲
Orthologous residues
SOX2 (human): Y125‑p, SOX2 (mouse): Y127‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry
 Relevant cell lines - cell types - tissues:  ES (stem)
 Cellular systems studied:  cell lines
 Species studied:  human

T126-p - SOX2 (human) ▲
Orthologous residues
SOX2 (human): T126‑p, SOX2 (mouse): T128‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry
 Relevant cell lines - cell types - tissues:  ES (stem)
 Cellular systems studied:  cell lines
 Species studied:  human

S138-p - SOX2 (human) ▲
Orthologous residues
SOX2 (human): S138‑p, SOX2 (mouse): S140‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry
 Relevant cell lines - cell types - tissues:  ES (stem)
 Cellular systems studied:  cell lines
 Species studied:  human

S249-p - SOX2 (human) ▲
Orthologous residues
SOX2 (human): S249‑p, SOX2 (mouse): S251‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry
 Relevant cell lines - cell types - tissues:  ES (stem)
 Cellular systems studied:  cell lines
 Species studied:  human

S250-p - SOX2 (human) ▲
Orthologous residues
SOX2 (human): S250‑p, SOX2 (mouse): S252‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry
 Relevant cell lines - cell types - tissues:  ES (stem)
 Cellular systems studied:  cell lines
 Species studied:  human

S251-p - SOX2 (human) ▲
Orthologous residues
SOX2 (human): S251‑p, SOX2 (mouse): S253‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry
 Relevant cell lines - cell types - tissues:  ES (stem)
 Cellular systems studied:  cell lines
 Species studied:  human


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