Curated Information
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Curated Information Page
PubMed Id: 22084245 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Zheng Y, Asara JM, Tyner AL (2012) Protein-tyrosine kinase 6 promotes peripheral adhesion complex formation and cell migration by phosphorylating p130 CRK-associated substrate. J Biol Chem 287, 148-58 22084245
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T308-p - Akt1 (human)
Orthologous residues
Akt1 (human): T308‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
 Disease tissue studied:  prostate cancer
 Relevant cell lines - cell types - tissues:  PC3 (prostate cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Brk (human) increase Brk induces and Brk siRNA inhibits

S473-p - Akt1 (human)
Orthologous residues
Akt1 (human): S473‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
 Disease tissue studied:  prostate cancer
 Relevant cell lines - cell types - tissues:  PC3 (prostate cell)
 Cellular systems studied:  cell lines
 Species studied:  human

T202-p - ERK1 (human)
Orthologous residues
ERK1 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
 Disease tissue studied:  prostate cancer
 Relevant cell lines - cell types - tissues:  PC3 (prostate cell)
 Cellular systems studied:  cell lines
 Species studied:  human

Y204-p - ERK1 (human)
Orthologous residues
ERK1 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
 Disease tissue studied:  prostate cancer
 Relevant cell lines - cell types - tissues:  PC3 (prostate cell)
 Cellular systems studied:  cell lines
 Species studied:  human

T185-p - ERK2 (human)
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
 Disease tissue studied:  prostate cancer
 Relevant cell lines - cell types - tissues:  PC3 (prostate cell)
 Cellular systems studied:  cell lines
 Species studied:  human

Y187-p - ERK2 (human)
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
 Disease tissue studied:  prostate cancer
 Relevant cell lines - cell types - tissues:  PC3 (prostate cell)
 Cellular systems studied:  cell lines
 Species studied:  human

T219-p - ERK5 (human)
Orthologous residues
ERK5 (human): T219‑p, ERK5 (mouse): T219‑p, ERK5 (rat): T219‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
 Disease tissue studied:  prostate cancer
 Relevant cell lines - cell types - tissues:  PC3 (prostate cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Brk (human) increase Brk induces and Brk siRNA inhibits

Y221-p - ERK5 (human)
Orthologous residues
ERK5 (human): Y221‑p, ERK5 (mouse): Y221‑p, ERK5 (rat): Y221‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
 Disease tissue studied:  prostate cancer
 Relevant cell lines - cell types - tissues:  PC3 (prostate cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Brk (human) increase Brk induces and Brk siRNA inhibits

Y165-p - P130Cas (human)
Orthologous residues
P130Cas (human): Y165‑p, P130Cas iso7 (human): Y183‑p, P130Cas iso8 (human): Y165‑p, P130Cas (mouse): Y169‑p, P130Cas (rat): Y263‑p, P130Cas iso2 (rat): Y169‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
 Disease tissue studied:  prostate cancer
 Relevant cell lines - cell types - tissues:  PC3 (prostate cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Brk (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Brk (human) transfection of constitutively active enzyme, transfection of inactive enzyme, transfection of wild-type enzyme, co-immunoprecipitation
Downstream Regulation
 Effect of modification (process):  carcinogenesis, induced, cell adhesion, induced, cell motility, induced, cytoskeletal reorganization
 Comments:  P130Cas phosphorylation is important in development of peripheral adhesion complexes, oncogenic signaling, and cell migration.

Y664-p - P130Cas (human)
Orthologous residues
P130Cas (human): Y664‑p, P130Cas iso7 (human): Y682‑p, P130Cas iso8 (human): Y664‑p, P130Cas (mouse): Y668‑p, P130Cas (rat): Y762‑p, P130Cas iso2 (rat): Y668‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
 Disease tissue studied:  prostate cancer
 Relevant cell lines - cell types - tissues:  PC3 (prostate cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Brk (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Brk (human) transfection of constitutively active enzyme, transfection of inactive enzyme, transfection of wild-type enzyme, co-immunoprecipitation
Downstream Regulation
 Effect of modification (process):  carcinogenesis, induced, cell adhesion, induced, cell motility, induced, cytoskeletal reorganization
 Comments:  P130Cas phosphorylation is important in development of peripheral adhesion complexes, oncogenic signaling, and cell migration.


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