Curated Information
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Curated Information Page
PubMed Id: 12048203 
Yuan ZQ, et al. (2002) Inhibition of JNK by cellular stress- and tumor necrosis factor alpha-induced AKT2 through activation of the NF kappa B pathway in human epithelial Cells. J Biol Chem 277, 29973-82 12048203
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
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S474-p - Akt2 (human)
Orthologous residues
Akt2 (human): S474‑p, Akt2 (mouse): S474‑p, Akt2 (rat): S474‑p
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  ovarian cancer
 Relevant cell lines - cell types - tissues:  OVCAR3 (ovarian)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-1 increase
heat shock increase
hypertonic buffer increase
UV increase
TNF increase

T23-p - IKKA (human)
Orthologous residues
IKKA (human): T23‑p, IKKA (mouse): T23‑p, IKKA (rat): T23‑p
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site
 Disease tissue studied:  ovarian cancer
 Relevant cell lines - cell types - tissues:  A2780 (ovarian), COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, monkey
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt2 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt2 (human) transfection of constitutively active enzyme, pharmacological activator of upstream enzyme, pharmacological inhibitor of upstream enzyme, transfection of dominant-negative enzyme
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, phosphorylation
 Effect of modification (process):  apoptosis, inhibited, transcription, altered
 Comments:  NFKB transcription, IKB-alpha phosphorylation (Ser32), JNK phosphorylation

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