Curated Information
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Curated Information Page
PubMed Id: 12048203 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Yuan ZQ, et al. (2002) Inhibition of JNK by cellular stress- and tumor necrosis factor alpha-induced AKT2 through activation of the NF kappa B pathway in human epithelial Cells. J Biol Chem 277, 29973-82 12048203
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S474-p - Akt2 (human)
Orthologous residues
Akt2 (human): S474‑p, Akt2 (mouse): S474‑p, Akt2 (rat): S474‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  ovarian cancer
 Relevant cell lines - cell types - tissues:  OVCAR3 (ovarian)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-1 increase
heat shock increase
hypertonic buffer increase
UV increase
TNF increase

T23-p - IKKA (human)
Orthologous residues
IKKA (human): T23‑p, IKKA (mouse): T23‑p, IKKA (rat): T23‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site
 Disease tissue studied:  ovarian cancer
 Relevant cell lines - cell types - tissues:  A2780 (ovarian), COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, monkey
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt2 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt2 (human) transfection of constitutively active enzyme, pharmacological inhibitor of upstream enzyme, transfection of dominant-negative enzyme, pharmacological activator of upstream enzyme
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, phosphorylation
 Effect of modification (process):  apoptosis, inhibited, transcription, altered
 Comments:  NFKB transcription, IKB-alpha phosphorylation (Ser32), JNK phosphorylation


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