Curated Information
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Curated Information Page
PubMed Id: 9363941 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Shieh SY, Ikeda M, Taya Y, Prives C (1997) DNA damage-induced phosphorylation of p53 alleviates inhibition by MDM2. Cell 91, 325-34 9363941
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S15-p - p53 (human)
Orthologous residues
p53 (human): S15‑p, p53 (mouse): S15‑p, p53 iso2 (mouse): S18‑p, p53 (rat): S15‑p, p53 (rabbit): S15‑p, p53 (monkey): S15‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  colorectal cancer, colorectal carcinoma, prostate cancer
 Relevant cell lines - cell types - tissues:  LNCaP (prostate cell), RKO (intestinal)
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
 Comments:  CB17 cells
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE DNAPK (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
camptothecin increase
UV increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, protein conformation
 Effect of modification (process):  transcription, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
MDM2 (human) Disrupts co-immunoprecipitation

S37-p - p53 (human)
Orthologous residues
p53 (human): S37‑p, p53 (mouse): S34‑p, p53 iso2 (mouse): S37‑p, p53 (rat): S39‑p, p53 (rabbit): N37‑p, p53 (monkey): S37‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE DNAPK (human)
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, protein conformation
 Effect of modification (process):  transcription, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
MDM2 (human) Disrupts co-immunoprecipitation


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