Curated Information
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Curated Information Page
PubMed Id: 18372248 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Wang L, Harris TE, Lawrence JC (2008) Regulation of proline-rich Akt substrate of 40 kDa (PRAS40) function by mammalian target of rapamycin complex 1 (mTORC1)-mediated phosphorylation. J Biol Chem 283, 15619-27 18372248
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S183-p - PRAS40 (human)
Orthologous residues
PRAS40 (human): S183‑p, PRAS40 iso3 (human): S203‑p, PRAS40 (mouse): S184‑p, PRAS40 (rat): S184‑p
Characterization
 Methods used to characterize site in vivo 2D analysis, [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE mTOR (mouse)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE mTOR (human) pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
rapamycin decrease

S202-p - PRAS40 (human)
Orthologous residues
PRAS40 (human): S202‑p, PRAS40 iso3 (human): S222‑p, PRAS40 (mouse): S203‑p, PRAS40 (rat): S203‑p
Characterization
 Methods used to characterize site in vivo 2D analysis, [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S203-p - PRAS40 (human)
Orthologous residues
PRAS40 (human): S203‑p, PRAS40 iso3 (human): S223‑p, PRAS40 (mouse): S204‑p, PRAS40 (rat): S204‑p
Characterization
 Methods used to characterize site in vivo 2D analysis, [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S212-p - PRAS40 (human)
Orthologous residues
PRAS40 (human): S212‑p, PRAS40 iso3 (human): S232‑p, PRAS40 (mouse): S213‑p, PRAS40 (rat): S213‑p
Characterization
 Methods used to characterize site in vivo 2D analysis, [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE mTOR (mouse)

S221-p - PRAS40 (human)
Orthologous residues
PRAS40 (human): S221‑p, PRAS40 iso3 (human): S241‑p, PRAS40 (mouse): S222‑p, PRAS40 (rat): S222‑p
Characterization
 Methods used to characterize site in vivo 2D analysis, [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE mTOR (mouse)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE mTOR (human) pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
rapamycin decrease
Downstream Regulation
 Effect of modification (function):  activity, inhibited, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 beta (human) Induces co-immunoprecipitation
 Comments:  decreases Akt1S1 inhibitory activity toward mTORC1

T246-p - PRAS40 (human)
Orthologous residues
PRAS40 (human): T246‑p, PRAS40 iso3 (human): T266‑p, PRAS40 (mouse): T247‑p, PRAS40 (rat): T247‑p
Characterization
 Methods used to characterize site in vivo 2D analysis, [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (mouse)
Downstream Regulation
 Effect of modification (function):  activity, inhibited, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 beta (human) Induces co-immunoprecipitation
 Comments:  decreases Akt1S1 inhibitory activity toward mTORC1


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