Curated Information
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PubMed Id: 10455013 
Casamayor A, Morrice NA, Alessi DR (1999) Phosphorylation of Ser-241 is essential for the activity of 3-phosphoinositide-dependent protein kinase-1: identification of five sites of phosphorylation in vivo. Biochem J 342 ( Pt 2), 287-92 10455013
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

S25-p - PDK1 (human)
Orthologous residues
PDK1 (human): S25‑p, PDK1 iso4 (human): S25‑p, PDK1 (mouse): S25‑p, PDK1 (rat): S25‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PDK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPP2CA (human)
KINASE PDK1 (human) transfection of wild-type enzyme in E.coli

S241-p - PDK1 (human)
Orthologous residues
PDK1 (human): S241‑p, PDK1 iso4 (human): S114‑p, PDK1 (mouse): S244‑p, PDK1 (rat): S244‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PDK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PDK1 (human) transfection of wild-type enzyme in E.coli
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced

S393-p - PDK1 (human)
Orthologous residues
PDK1 (human): S393‑p, PDK1 iso4 (human): S266‑p, PDK1 (mouse): S396‑p, PDK1 (rat): S396‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PDK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PDK1 (human) transfection of wild-type enzyme in E.coli
PHOSPHATASE PPP2CA (human)

S396-p - PDK1 (human)
Orthologous residues
PDK1 (human): S396‑p, PDK1 iso4 (human): S269‑p, PDK1 (mouse): S399‑p, PDK1 (rat): S399‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PDK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PDK1 (human) transfection of wild-type enzyme in E.coli
PHOSPHATASE PPP2CA (human)

S410-p - PDK1 (human)
Orthologous residues
PDK1 (human): S410‑p, PDK1 iso4 (human): S283‑p, PDK1 (mouse): S413‑p, PDK1 (rat): S413‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PDK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPP2CA (human)
KINASE PDK1 (human) transfection of wild-type enzyme in E.coli


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