Mayo LD, Donner DB (2001) A phosphatidylinositol 3-kinase/Akt pathway promotes translocation of Mdm2 from the cytoplasm to the nucleus. Proc Natl Acad Sci U S A 98, 11598-603 11504915

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S166-p - MDM2 (human) ▲

Modsite: SsRRRAIsEtEENsD SwissProt Entrez-Gene Orthologous residues MDM2 (human): S166‑p, MDM2 (mouse): S163‑p, MDM2 (rat): S138‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site Relevant cell lines - cell types - tissues:  293 (epithelial), MCF-7 (breast cell), Saos-2 (bone cell) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE Akt1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Akt1 (human) pharmacological inhibitor of upstream enzyme, transfection of constitutively active enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes IGF-1 increase Downstream Regulation Effect of modification (function):  intracellular localization

S186-p - MDM2 (human) ▲

Modsite: RQRkRHksDsIsLsF SwissProt Entrez-Gene Orthologous residues MDM2 (human): S186‑p, MDM2 (mouse): S183‑p, MDM2 (rat): A158‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site Relevant cell lines - cell types - tissues:  293 (epithelial), MCF-7 (breast cell), Saos-2 (bone cell) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE Akt1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Akt1 (human) pharmacological inhibitor of upstream enzyme, transfection of constitutively active enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes IGF-1 increase Downstream Regulation Effect of modification (function):  intracellular localization