Curated Information
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PubMed Id: 8816475 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Schlaepfer DD, Hunter T (1996) Evidence for in vivo phosphorylation of the Grb2 SH2-domain binding site on focal adhesion kinase by Src-family protein-tyrosine kinases. Mol Cell Biol 16, 5623-33 8816475
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y397-p - FAK iso3 (mouse) ▲
Orthologous residues
FAK (human): Y397‑p, FAK iso2 (human): Y216‑p, FAK iso5 (human): Y397‑p, FAK (mouse): Y428‑p, FAK iso3 (mouse): Y397‑p, FAK iso4 (mouse): Y397‑p, FAK (rat): Y397‑p, FAK (chicken): Y397‑p, FAK iso5 (chicken): ‑
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phosphoamino acid analysis, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fibronectin increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Src (human) Induces electrophoretic visualization, co-immunoprecipitation, in vitro
Grb2 (human) SH2 Induces electrophoretic visualization, co-immunoprecipitation, in vitro

Y407-p - FAK iso3 (mouse) ▲
Orthologous residues
FAK (human): Y407‑p, FAK iso2 (human): Y226‑p, FAK iso5 (human): Y407‑p, FAK (mouse): Y438‑p, FAK iso3 (mouse): Y407‑p, FAK iso4 (mouse): Y407‑p, FAK (rat): Y407‑p, FAK (chicken): Y407‑p, FAK iso5 (chicken): ‑
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phosphoamino acid analysis, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fibronectin increase

Y576-p - FAK iso3 (mouse) ▲
Orthologous residues
FAK (human): Y576‑p, FAK iso2 (human): Y424‑p, FAK iso5 (human): Y576‑p, FAK (mouse): Y614‑p, FAK iso3 (mouse): Y576‑p, FAK iso4 (mouse): Y576‑p, FAK (rat): Y576‑p, FAK (chicken): Y576‑p, FAK iso5 (chicken): ‑
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phosphoamino acid analysis, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fibronectin increase

Y577-p - FAK iso3 (mouse) ▲
Orthologous residues
FAK (human): Y577‑p, FAK iso2 (human): Y425‑p, FAK iso5 (human): Y577‑p, FAK (mouse): Y615‑p, FAK iso3 (mouse): Y577‑p, FAK iso4 (mouse): Y577‑p, FAK (rat): Y577‑p, FAK (chicken): Y577‑p, FAK iso5 (chicken): ‑
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phosphoamino acid analysis, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fibronectin increase

Y925-p - FAK iso3 (mouse) ▲
Orthologous residues
FAK (human): Y925‑p, FAK iso2 (human): Y752‑p, FAK iso5 (human): Y938‑p, FAK (mouse): Y963‑p, FAK iso3 (mouse): Y925‑p, FAK iso4 (mouse): Y928‑p, FAK (rat): Y928‑p, FAK (chicken): Y926‑p, FAK iso5 (chicken): Y232‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phosphoamino acid analysis, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout]
 Cellular systems studied:  cell lines
 Species studied:  human, mouse
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Src (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (human)
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fibronectin increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Grb2 (human) SH2 Induces electrophoretic visualization, co-immunoprecipitation, in vitro


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