Curated Information
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Curated Information Page
PubMed Id: 20356833 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Lou H, et al. (2010) Serine 129 phosphorylation reduces the ability of alpha-synuclein to regulate tyrosine hydroxylase and protein phosphatase 2A in vitro and in vivo. J Biol Chem 285, 17648-61 20356833
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S129-p - SNCA (human) ▲
Orthologous residues
SNCA (human): S129‑p, SNCA (mouse): S129‑p, SNCA (rat): S129‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MN9D ('neuron, mesencephalic')
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE PLK2 (human)

S19-p - TH (mouse) ▲
Orthologous residues
TH (human): S19‑p, TH iso2 (human): S19‑p, TH iso3 (human): S19‑p, TH iso5 (human): S19‑p, TH (mouse): S19‑p, TH (rat): S19‑p, TH (cow): S19‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MN9D ('neuron, mesencephalic')
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPP2CA (human) transfection of wild-type enzyme, pharmacological inhibitor of upstream enzyme, phospho-antibody
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
okadaic acid increase
fostreicin increase
SNCA (human) decrease
SNCA (human) decrease SNCA KO induces
SNCA (human) augment treatment-induced decrease S129A SNCA mutant
SNCA (human) decrease in dopaminergic tissues
Downstream Regulation
 Effect of modification (function):  phosphorylation
 Comments:  SNCA S129A mutant has higher PPP2CA activation inhibiting TH S19 phosphorylation.

S40-p - TH (mouse) ▲
Orthologous residues
TH (human): S71‑p, TH iso2 (human): S67‑p, TH iso3 (human): S40‑p, TH iso5 (human): S44‑p, TH (mouse): S40‑p, TH (rat): S40‑p, TH (cow): S40‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MN9D ('neuron, mesencephalic')
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SNCA (human) decrease in dopaminergic tissues


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