Curated Information
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Curated Information Page
PubMed Id: 20308429 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Pasapera AM, et al. (2010) Myosin II activity regulates vinculin recruitment to focal adhesions through FAK-mediated paxillin phosphorylation. J Cell Biol 188, 877-90 20308429
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y428-p - FAK (mouse) ▲
Orthologous residues
FAK (human): Y397‑p, FAK iso2 (human): Y216‑p, FAK iso5 (human): Y397‑p, FAK (mouse): Y428‑p, FAK iso3 (mouse): Y397‑p, FAK iso4 (mouse): Y397‑p, FAK (rat): Y397‑p, FAK (chicken): Y397‑p, FAK iso5 (chicken): ‑
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
blebbistatin decrease
PP2 blebbistatin inhibit treatment-induced decrease
PF271 blebbistatin no effect upon treatment-induced decrease
PF271, PP2 blebbistatin no effect upon treatment-induced decrease

Y31-p - PXN (mouse) ▲
Orthologous residues
PXN (human): Y31‑p, PXN iso2 (human): Y31‑p, PXN iso3 (human): Y31‑p, PXN iso6 (human): Y38‑p, PXN (mouse): Y31‑p, PXN iso2 (mouse): Y31‑p, PXN (rat): Y31‑p, PXN (chicken): Y31‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
blebbistatin decrease
PP2 blebbistatin augment treatment-induced decrease
PF271 blebbistatin augment treatment-induced decrease
PF271, PP2 blebbistatin augment treatment-induced decrease
vanadate blebbistatin inhibit treatment-induced decrease
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell adhesion, induced
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
vinculin (mouse) Induces co-immunoprecipitation
 Comments:  Paxillin Y31/118 phosphorylation promotes paxillin-vinculin interaction and vinculin recruitment to focal adhesions.

Y118-p - PXN (mouse) ▲
Orthologous residues
PXN (human): Y118‑p, PXN iso2 (human): Y118‑p, PXN iso3 (human): Y118‑p, PXN iso6 (human): Y125‑p, PXN (mouse): Y118‑p, PXN iso2 (mouse): Y118‑p, PXN (rat): Y118‑p, PXN (chicken): Y118‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
blebbistatin decrease
PP2 blebbistatin no effect upon treatment-induced decrease
PF271 blebbistatin no effect upon treatment-induced decrease
PF271, PP2 blebbistatin no effect upon treatment-induced decrease
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell adhesion, induced
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
vinculin (mouse) Induces co-immunoprecipitation
 Comments:  Paxillin Y31/118 phosphorylation promotes paxillin-vinculin interaction and vinculin recruitment to focal adhesions.

Y527-p - Src (mouse) ▲
Orthologous residues
Src (human): Y522‑p, Src (mouse): Y527‑p, Src iso2 (mouse): Y521‑p, Src (rat): Y522‑p, Src (chicken): Y519‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
blebbistatin no change compared to control
PP2 blebbistatin decrease
PF271 blebbistatin no change compared to control
augment treatment-induced decrease
PF271, PP2 no change compared to control


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