Curated Information
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Curated Information Page
PubMed Id: 22037600 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Iwasaki H, et al. (2011) The IκB kinase complex regulates the stability of cytokine-encoding mRNA induced by TLR-IL-1R by controlling degradation of regnase-1. Nat Immunol 12, 1167-75 22037600
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S435-p - MCPIP1 (mouse)
Orthologous residues
MCPIP1 (human): S438‑p, MCPIP1 (mouse): S435‑p, MCPIP1 (rat): S435‑p
Characterization
 Methods used to characterize site in vivo electrophoretic mobility shift, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical), Rat1 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, rat
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE IKKB (human) transfection of wild-type enzyme, modification site within consensus motif, co-immunoprecipitation, pharmacological inhibitor of upstream enzyme, genetic knockout/knockin of upstream enzyme, transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase
IL-1b increase
TNF increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, protein degradation, ubiquitination
 Effect of modification (process):  RNA stability, induced
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
BTRC (human) Induces co-immunoprecipitation
 Comments:  phosphorylation followed by degradation of ZC3H12A (aka regnase-1) augments IL-6 mRNA stability

S439-p - MCPIP1 (mouse)
Orthologous residues
MCPIP1 (human): S442‑p, MCPIP1 (mouse): S439‑p, MCPIP1 (rat): S439‑p
Characterization
 Methods used to characterize site in vivo electrophoretic mobility shift, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical), Rat1 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  human, rat
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE IKKB (human) transfection of wild-type enzyme, modification site within consensus motif, co-immunoprecipitation, pharmacological inhibitor of upstream enzyme, genetic knockout/knockin of upstream enzyme, transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase
IL-1b increase
TNF increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, protein degradation, ubiquitination
 Effect of modification (process):  RNA stability, induced
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
BTRC (human) Induces co-immunoprecipitation
 Comments:  phosphorylation followed by degradation of ZC3H12A (aka regnase-1) augments IL-6 mRNA stability


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