Curated Information
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Curated Information Page
PubMed Id: 22094256 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Anders L, et al. (2011) A systematic screen for CDK4/6 substrates links FOXM1 phosphorylation to senescence suppression in cancer cells. Cancer Cell 20, 620-34 22094256
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

S4-p - FOXM1 (human)
Orthologous residues
FOXM1 (human): S4‑p, FOXM1 iso2 (human): S4‑p, FOXM1 (mouse): S4‑p
Characterization
 Methods used to characterize site in vivo electrophoretic mobility shift, immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  HeLa (cervical), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CDK4 (human) modification site within consensus motif, phosphopeptide analysis, electrophoretic mobility shift
 Comments:  CDK4/6 phosphorylated sites

S35-p - FOXM1 (human)
Orthologous residues
FOXM1 (human): S35‑p, FOXM1 iso2 (human): S35‑p, FOXM1 (mouse): S35‑p
Characterization
 Methods used to characterize site in vivo electrophoretic mobility shift, immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  HeLa (cervical), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CDK4 (human) modification site within consensus motif, phosphopeptide analysis, electrophoretic mobility shift
 Comments:  CDK4/6 phosphorylated sites

S331-p - FOXM1 (human)
Orthologous residues
FOXM1 (human): S331‑p, FOXM1 iso2 (human): , FOXM1 (mouse): S329‑p
Characterization
 Methods used to characterize site in vivo electrophoretic mobility shift, immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  HeLa (cervical), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human

S451-p - FOXM1 (human)
Orthologous residues
FOXM1 (human): S451‑p, FOXM1 iso2 (human): S436‑p, FOXM1 (mouse): L450‑p
Characterization
 Methods used to characterize site in vivo electrophoretic mobility shift, immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  HeLa (cervical), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK4 (human)
KINASE CDK6 (human)

S489-p - FOXM1 (human)
Orthologous residues
FOXM1 (human): S489‑p, FOXM1 iso2 (human): S474‑p, FOXM1 (mouse): S488‑p
Characterization
 Methods used to characterize site in vivo electrophoretic mobility shift, immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  HeLa (cervical), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK4 (human)
KINASE CDK6 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CDK4 (human) modification site within consensus motif, phosphopeptide analysis, electrophoretic mobility shift
 Comments:  CDK4/6 phosphorylated sites

S508-p - FOXM1 (human)
Orthologous residues
FOXM1 (human): S508‑p, FOXM1 iso2 (human): S493‑p, FOXM1 (mouse): S507‑p
Characterization
 Methods used to characterize site in vivo electrophoretic mobility shift, immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  HeLa (cervical), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK6 (human)
KINASE CDK4 (human)

T510-p - FOXM1 (human)
Orthologous residues
FOXM1 (human): T510‑p, FOXM1 iso2 (human): T495‑p, FOXM1 (mouse): T509‑p
Characterization
 Methods used to characterize site in vivo electrophoretic mobility shift, immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  HeLa (cervical), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK4 (human)
KINASE CDK6 (human)

S522-p - FOXM1 (human)
Orthologous residues
FOXM1 (human): S522‑p, FOXM1 iso2 (human): S507‑p, FOXM1 (mouse): S521‑p
Characterization
 Methods used to characterize site in vivo electrophoretic mobility shift, immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  HeLa (cervical), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK6 (human)
KINASE CDK4 (human)

T600-p - FOXM1 (human)
Orthologous residues
FOXM1 (human): T600‑p, FOXM1 iso2 (human): T585‑p, FOXM1 (mouse): T597‑p
Characterization
 Methods used to characterize site in vivo electrophoretic mobility shift, immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  HeLa (cervical), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK4 (human)
KINASE CDK6 (human)
Downstream Regulation
 Effect of modification (function):  protein stabilization
 Effect of modification (process):  transcription, induced

T611-p - FOXM1 (human)
Orthologous residues
FOXM1 (human): T611‑p, FOXM1 iso2 (human): T596‑p, FOXM1 (mouse): T608‑p
Characterization
 Methods used to characterize site in vivo electrophoretic mobility shift, immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  HeLa (cervical), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK4 (human)
KINASE CDK6 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CDK4 (human) modification site within consensus motif, phosphopeptide analysis, electrophoretic mobility shift
 Comments:  CDK4/6 phosphorylated sites
Downstream Regulation
 Effect of modification (function):  protein stabilization
 Effect of modification (process):  transcription, induced

T620-p - FOXM1 (human)
Orthologous residues
FOXM1 (human): T620‑p, FOXM1 iso2 (human): T605‑p, FOXM1 (mouse): D617‑p
Characterization
 Methods used to characterize site in vivo electrophoretic mobility shift, immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  HeLa (cervical), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK4 (human)
KINASE CDK6 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CDK4 (human) modification site within consensus motif, phosphopeptide analysis, electrophoretic mobility shift
 Comments:  CDK4/6 phosphorylated sites
Downstream Regulation
 Effect of modification (function):  protein stabilization
 Effect of modification (process):  transcription, induced

T627-p - FOXM1 (human)
Orthologous residues
FOXM1 (human): T627‑p, FOXM1 iso2 (human): T612‑p, FOXM1 (mouse): T624‑p
Characterization
 Methods used to characterize site in vivo electrophoretic mobility shift, immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  HeLa (cervical), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK6 (human)
KINASE CDK4 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CDK4 (human) modification site within consensus motif, phosphopeptide analysis, electrophoretic mobility shift
 Comments:  CDK4/6 phosphorylated sites
Downstream Regulation
 Effect of modification (function):  protein stabilization
 Effect of modification (process):  transcription, induced

S638-p - FOXM1 (human)
Orthologous residues
FOXM1 (human): S638‑p, FOXM1 iso2 (human): S623‑p, FOXM1 (mouse): S635‑p
Characterization
 Methods used to characterize site in vivo electrophoretic mobility shift, immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  HeLa (cervical), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  protein stabilization
 Effect of modification (process):  transcription, induced

S672-p - FOXM1 (human)
Orthologous residues
FOXM1 (human): S672‑p, FOXM1 iso2 (human): S657‑p, FOXM1 (mouse): S669‑p
Characterization
 Methods used to characterize site in vivo electrophoretic mobility shift, immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  HeLa (cervical), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  protein stabilization
 Effect of modification (process):  transcription, induced

S704-p - FOXM1 (human)
Orthologous residues
FOXM1 (human): S704‑p, FOXM1 iso2 (human): S689‑p, FOXM1 (mouse): S701‑p
Characterization
 Methods used to characterize site in vivo electrophoretic mobility shift, immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  HeLa (cervical), U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK6 (human)
KINASE CDK4 (human)
Downstream Regulation
 Effect of modification (function):  protein stabilization
 Effect of modification (process):  transcription, induced


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