|
Orthologous residues
|
|
ARRB1 (human): S412‑p, ARRB1 iso2 (human): S404‑p, ARRB1 (mouse): S412‑p, ARRB1 (rat): S412‑p, ARRB1 (cow): S412‑p
|
|
Characterization
|
|
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
|
|
Relevant cell lines - cell types - tissues:
293 (epithelial) [ADRB2 (human)], 293 (epithelial) [ARRB1 (human)], 293 (epithelial) [MEK1 (human)]
|
|
Cellular systems studied:
cell lines
|
|
Species studied:
human
|
|
Enzymes shown to modify site in vitro:
|
|
|
|
Upstream Regulation
|
|
Potential in vivo enzymes for site:
|
|
Type
|
Enzyme
|
Evidence
|
Notes
|
|
KINASE
|
ERK2 (human)
|
transfection of constitutively active enzyme, transfection of inactive enzyme
|
constitutively active and dominant-negative MEK1 constructs have been used in this study
|
|
|
Downstream Regulation
|
|
Effect of modification (function):
inhibition, molecular association, regulation
|
|
Modification regulates interactions with:
|
|
Interacting molecule
|
Interacting domains
|
Effect
|
Consequences (function)
|
Consequences (process)
|
Detection assays
|
|
ADRB2 (human)
|
|
Disrupts
|
receptor internalization, altered
|
|
co-immunoprecipitation
|
|
