Curated Information
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Curated Information Page
PubMed Id: 16899510 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Tsuji T, Ficarro SB, Jiang W (2006) Essential role of phosphorylation of MCM2 by Cdc7/Dbf4 in the initiation of DNA replication in mammalian cells. Mol Biol Cell 17, 4459-72 16899510
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S27-p - MCM2 (human) ▲
Orthologous residues
MCM2 (human): S27‑p, MCM2 (mouse): S27‑p, MCM2 (rat): S28‑p
Characterization
 Methods used to characterize site in vivo:  2D analysis, [32P] bio-synthetic labeling, immunoprecipitation, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE CDC7 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CDK7 (human) siRNA inhibition of enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
roscovitine decrease
olomoucine decrease
siRNA decrease
Downstream Regulation
 Effect of modification (function):  activation, molecular association, regulation
 Effect of modification (process):  cell cycle regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
DNA Not reported
 Comments:  regulates ATPase activity in vitro

S41-p - MCM2 (human) ▲
Orthologous residues
MCM2 (human): S41‑p, MCM2 (mouse): S41‑p, MCM2 (rat): S42‑p
Characterization
 Methods used to characterize site in vivo:  2D analysis, [32P] bio-synthetic labeling, immunoprecipitation, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE CDC7 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CDK7 (human) siRNA inhibition of enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
roscovitine decrease
olomoucine decrease
siRNA decrease
Downstream Regulation
 Effect of modification (function):  activation, molecular association, regulation
 Effect of modification (process):  cell cycle regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
DNA Not reported
 Comments:  regulates ATPase activity in vitro

S139-p - MCM2 (human) ▲
Orthologous residues
MCM2 (human): S139‑p, MCM2 (mouse): S139‑p, MCM2 (rat): S140‑p
Characterization
 Methods used to characterize site in vivo:  2D analysis, [32P] bio-synthetic labeling, immunoprecipitation, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE CDC7 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CDK7 (human) siRNA inhibition of enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
roscovitine decrease
olomoucine decrease
siRNA decrease
Downstream Regulation
 Effect of modification (function):  activation, molecular association, regulation
 Effect of modification (process):  cell cycle regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
DNA Not reported
 Comments:  regulates ATPase activity in vitro


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