Curated Information
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PubMed Id: 18250158 
Cheng H, Ross JA, Frost JA, Kirken RA (2008) Phosphorylation of human jak3 at tyrosines 904 and 939 positively regulates its activity. Mol Cell Biol 28, 2271-82 18250158
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y785-p - JAK3 (human)
Orthologous residues
JAK3 (human): Y785‑p, JAK3 (mouse): Y781‑p, JAK3 iso2 (mouse): Y1000‑p, JAK3 iso3 (mouse): Y996‑p, JAK3 (rat): Y781‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JAK3 (human) mutation in upstream enzyme recognition motif

Y904-p - JAK3 (human)
Orthologous residues
JAK3 (human): Y904‑p, JAK3 (mouse): Y900‑p, JAK3 iso2 (mouse): Y1119‑p, JAK3 iso3 (mouse): Y1113‑p, JAK3 (rat): Y900‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
 Disease tissue studied:  leukemia, T cell leukemia
 Relevant cell lines - cell types - tissues:  Kit225 (T lymphocyte), lymphocyte, YT
 Cellular systems studied:  cell lines, primary cells
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE JAK3 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JAK3 (human) mutation in upstream enzyme recognition motif
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IL-2 increase
IL-9 increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, phosphorylation
 Comments:  required for STAT5A Y694 phosphorylation and its further transcriptional activation

Y939-p - JAK3 (human)
Orthologous residues
JAK3 (human): Y939‑p, JAK3 (mouse): Y935‑p, JAK3 iso2 (mouse): Y1154‑p, JAK3 iso3 (mouse): Y1148‑p, JAK3 (rat): Y935‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
 Disease tissue studied:  leukemia, T cell leukemia
 Relevant cell lines - cell types - tissues:  Kit225 (T lymphocyte), lymphocyte, YT
 Cellular systems studied:  cell lines, primary cells
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE JAK3 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JAK3 (human) mutation in upstream enzyme recognition motif
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IL-2 increase
IL-9 increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, molecular association, regulation, phosphorylation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
STAT5A (human) Induces pull-down assay
 Comments:  possible docking site for kinase substrates; required for STAT5A Y694 phosphorylation and its further transcriptional activation

Y980-p - JAK3 (human)
Orthologous residues
JAK3 (human): Y980‑p, JAK3 (mouse): Y976‑p, JAK3 iso2 (mouse): Y1195‑p, JAK3 iso3 (mouse): Y1189‑p, JAK3 (rat): Y976‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JAK3 (human) mutation in upstream enzyme recognition motif

Y981-p - JAK3 (human)
Orthologous residues
JAK3 (human): Y981‑p, JAK3 (mouse): Y977‑p, JAK3 iso2 (mouse): Y1196‑p, JAK3 iso3 (mouse): Y1190‑p, JAK3 (rat): Y977‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JAK3 (human) mutation in upstream enzyme recognition motif

Y694-p - STAT5A (human)
Orthologous residues
STAT5A (human): Y694‑p, STAT5A (mouse): Y694‑p, STAT5A iso2 (mouse): , STAT5A (rat): Y694‑p, STAT5A (fruit fly): Y711‑p, STAT5A (sheep): Y694‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, phospho-antibody
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JAK3 (human) mutation in upstream enzyme recognition motif
Downstream Regulation
 Effect of modification (function):  activity, induced
 Effect of modification (process):  transcription, altered


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