Curated Information
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Curated Information Page
PubMed Id: 18286467 
Charych DH, et al. (2008) Inhibition of Cdc7/Dbf4 kinase activity affects specific phosphorylation sites on MCM2 in cancer cells. J Cell Biochem 104, 1075-86 18286467
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S31-p - MCM2 (human)
Orthologous residues
MCM2 (human): S31‑p, MCM2 iso4 (human): S31‑p, MCM2 (mouse): S31‑p, MCM2 (rat): S32‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDC7 (human)

S40-p - MCM2 (human)
Orthologous residues
MCM2 (human): S40‑p, MCM2 iso4 (human): S40‑p, MCM2 (mouse): S40‑p, MCM2 (rat): S41‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody
 Disease tissue studied:  lung cancer
 Relevant cell lines - cell types - tissues:  A549 (pulmonary)
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDC7 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CDC7 (human) siRNA inhibition of enzyme siRNA knockdown CDC7 or its cofactor Dbf4
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
siRNA decrease

S53-p - MCM2 (human)
Orthologous residues
MCM2 (human): S53‑p, MCM2 iso4 (human): S53‑p, MCM2 (mouse): S53‑p, MCM2 (rat): S54‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDC7 (human)

S108-p - MCM2 (human)
Orthologous residues
MCM2 (human): S108‑p, MCM2 iso4 (human): S108‑p, MCM2 (mouse): S108‑p, MCM2 (rat): S109‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody
 Disease tissue studied:  lung cancer
 Relevant cell lines - cell types - tissues:  A549 (pulmonary)
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDC7 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CDC7 (human) siRNA inhibition of enzyme siRNA knockdown CDC7 or its cofactor Dbf4
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
siRNA decrease

S139-p - MCM2 (human)
Orthologous residues
MCM2 (human): S139‑p, MCM2 iso4 (human): S120‑p, MCM2 (mouse): S139‑p, MCM2 (rat): S140‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDC7 (human)

S220-p - MCM2 (human)
Orthologous residues
MCM2 (human): S220‑p, MCM2 iso4 (human): S201‑p, MCM2 (mouse): S220‑p, MCM2 (rat): S221‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDC7 (human)


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