Curated Information
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Curated Information Page
PubMed Id: 18308725 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Matitau AE, Scheid MP (2008) Phosphorylation of MEKK3 at threonine 294 promotes 14-3-3 association to inhibit nuclear factor kappaB activation. J Biol Chem 283, 13261-8 18308725
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T294-p - MEKK3 (human)
Orthologous residues
MEKK3 (human): T294‑p, MEKK3 (mouse): T294‑p, MEKK3 (rat): T294‑p
Characterization
 Methods used to characterize site in vivo 2D analysis, [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  293 (epithelial), RAW 264.7 (macrophage)
 Comments:  constitutive phosphorylation
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS decrease
TNF decrease
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 beta (human) Induces co-immunoprecipitation
 Comments:  facilitated by phosphorylation of S526

S526-p - MEKK3 (human)
Orthologous residues
MEKK3 (human): S526‑p, MEKK3 (mouse): S526‑p, MEKK3 (rat): S526‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
okadaic acid increase
LPS increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced
 Comments:  necessary for MEKK3 T294 phosphorylation and its interaction with 14-3-3beta


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