Curated Information

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PubMed Id: 18308725

This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus^®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus^®, click here.

Matitau AE, Scheid MP (2008) Phosphorylation of MEKK3 at threonine 294 promotes 14-3-3 association to inhibit nuclear factor kappaB activation. J Biol Chem 283, 13261-8 18308725

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T294-p - MEKK3 (human)

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Orthologous residues

MEKK3 (human): T294‑p, MEKK3 (mouse): T294‑p, MEKK3 (rat): T294‑p

Characterization

Methods used to characterize site in vivo: 2D analysis, [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody

Relevant cell lines - cell types - tissues: 293 (epithelial), RAW 264.7 (macrophage)

Comments: constitutive phosphorylation

Upstream Regulation

Treatments, proteins and their effect on site modification:

Treatments	Referenced Treatments	Manipulated Protein	Referenced Protein	Effect	Notes
LPS				decrease
TNF				decrease

Downstream Regulation

Effect of modification (function): molecular association, regulation

Modification regulates interactions with:

Interacting molecule	Interacting domains	Effect	Consequences (function)	Consequences (process)	Detection assays
14-3-3 beta (human)		Induces			co-immunoprecipitation

Comments: facilitated by phosphorylation of S526

S526-p - MEKK3 (human)

▲

Orthologous residues

MEKK3 (human): S526‑p, MEKK3 (mouse): S526‑p, MEKK3 (rat): S526‑p

Characterization

Methods used to characterize site in vivo: immunoprecipitation, mutation of modification site, phospho-antibody

Relevant cell lines - cell types - tissues: 293 (epithelial)

Cellular systems studied: cell lines

Species studied: human

Upstream Regulation

Treatments, proteins and their effect on site modification:

Treatments	Referenced Treatments	Manipulated Protein	Referenced Protein	Effect	Notes
okadaic acid				increase
LPS				increase

Downstream Regulation

Effect of modification (function): enzymatic activity, induced

Comments: necessary for MEKK3 T294 phosphorylation and its interaction with 14-3-3beta

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