Curated Information
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Curated Information Page
PubMed Id: 22017877 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Duan S, et al. (2011) mTOR generates an auto-amplification loop by triggering the βTrCP- and CK1α-dependent degradation of DEPTOR. Mol Cell 44, 317-24 22017877
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S279-p - Deptor (human)
Orthologous residues
Deptor (human): S279‑p, Deptor (mouse): S279‑p, Deptor (rat): S386‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Disease tissue studied:  brain cancer, glioblastoma multiforme, glioma
 Relevant cell lines - cell types - tissues:  293T (epithelial), T98G (glial)
 Cellular systems studied:  cell lines
 Species studied:  human

S280-p - Deptor (human)
Orthologous residues
Deptor (human): S280‑p, Deptor (mouse): S280‑p, Deptor (rat): S387‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Disease tissue studied:  brain cancer, glioblastoma multiforme, glioma
 Relevant cell lines - cell types - tissues:  293T (epithelial), T98G (glial)
 Cellular systems studied:  cell lines
 Species studied:  human

S286-p - Deptor (human)
Orthologous residues
Deptor (human): S286‑p, Deptor (mouse): S286‑p, Deptor (rat): S393‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Disease tissue studied:  brain cancer, glioblastoma multiforme, glioma
 Relevant cell lines - cell types - tissues:  293T (epithelial), HeLa (cervical), T98G (glial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK1A (human) pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum increase
PP242 serum inhibit treatment-induced increase
D4476 serum inhibit treatment-induced increase
mTOR (human) increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, protein degradation, ubiquitination
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
BTRC (human) Induces co-immunoprecipitation

S287-p - Deptor (human)
Orthologous residues
Deptor (human): S287‑p, Deptor (mouse): S287‑p, Deptor (rat): S394‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Disease tissue studied:  brain cancer, glioblastoma multiforme, glioma
 Relevant cell lines - cell types - tissues:  293T (epithelial), HeLa (cervical), T98G (glial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK1A (human) pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum increase
PP242 serum inhibit treatment-induced increase
D4476 serum inhibit treatment-induced increase
mTOR (human) increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, protein degradation, ubiquitination
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
BTRC (human) Induces co-immunoprecipitation

S291-p - Deptor (human)
Orthologous residues
Deptor (human): S291‑p, Deptor (mouse): S291‑p, Deptor (rat): S398‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Disease tissue studied:  brain cancer, glioblastoma multiforme, glioma
 Relevant cell lines - cell types - tissues:  293T (epithelial), HeLa (cervical), T98G (glial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK1A (human) pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum increase
PP242 serum inhibit treatment-induced increase
D4476 serum inhibit treatment-induced increase
mTOR (human) increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, protein degradation, ubiquitination
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
BTRC (human) Induces co-immunoprecipitation

S293-p - Deptor (human)
Orthologous residues
Deptor (human): S293‑p, Deptor (mouse): S293‑p, Deptor (rat): S400‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Disease tissue studied:  brain cancer, glioblastoma multiforme, glioma
 Relevant cell lines - cell types - tissues:  293T (epithelial), T98G (glial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE mTOR (human) pharmacological inhibitor of upstream enzyme
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
BTRC (human) Induces co-immunoprecipitation

T295-p - Deptor (human)
Orthologous residues
Deptor (human): T295‑p, Deptor (mouse): T295‑p, Deptor (rat): T402‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Disease tissue studied:  brain cancer, glioblastoma multiforme, glioma
 Relevant cell lines - cell types - tissues:  293T (epithelial), T98G (glial)
 Cellular systems studied:  cell lines
 Species studied:  human

S297-p - Deptor (human)
Orthologous residues
Deptor (human): S297‑p, Deptor (mouse): S297‑p, Deptor (rat): S404‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Disease tissue studied:  brain cancer, glioblastoma multiforme, glioma
 Relevant cell lines - cell types - tissues:  293T (epithelial), T98G (glial)
 Cellular systems studied:  cell lines
 Species studied:  human

S298-p - Deptor (human)
Orthologous residues
Deptor (human): S298‑p, Deptor (mouse): S298‑p, Deptor (rat): S405‑p

S299-p - Deptor (human)
Orthologous residues
Deptor (human): S299‑p, Deptor (mouse): S299‑p, Deptor (rat): S406‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  brain cancer, glioblastoma multiforme, glioma
 Relevant cell lines - cell types - tissues:  293T (epithelial), T98G (glial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE mTOR (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE mTOR (human) pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum increase
PP242 serum inhibit treatment-induced increase
D4476 serum no effect upon treatment-induced increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
BTRC (human) Induces co-immunoprecipitation


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