Curated Information
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PubMed Id: 22017875 
Gao D, et al. (2011) mTOR drives its own activation via SCF(╬▓TrCP)-dependent degradation of the mTOR inhibitor DEPTOR. Mol Cell 44, 290-303 22017875
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
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S6-p - DEPTOR (human)
Orthologous residues
DEPTOR (human): S6‑p, DEPTOR (mouse): S6‑p, DEPTOR (rat): A18‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

T7-p - DEPTOR (human)
Orthologous residues
DEPTOR (human): T7‑p, DEPTOR (mouse): G7‑p, DEPTOR (rat): A19‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

T149-p - DEPTOR (human)
Orthologous residues
DEPTOR (human): T149‑p, DEPTOR (mouse): T149‑p, DEPTOR (rat): T256‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry (in vitro)
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK1A (human)

S168-p - DEPTOR (human)
Orthologous residues
DEPTOR (human): S168‑p, DEPTOR (mouse): S168‑p, DEPTOR (rat): S275‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

S235-p - DEPTOR (human)
Orthologous residues
DEPTOR (human): S235‑p, DEPTOR (mouse): S235‑p, DEPTOR (rat): S342‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

S244-p - DEPTOR (human)
Orthologous residues
DEPTOR (human): S244‑p, DEPTOR (mouse): S244‑p, DEPTOR (rat): S351‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

T259-p - DEPTOR (human)
Orthologous residues
DEPTOR (human): T259‑p, DEPTOR (mouse): T259‑p, DEPTOR (rat): T366‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

S260-p - DEPTOR (human)
Orthologous residues
DEPTOR (human): S260‑p, DEPTOR (mouse): S260‑p, DEPTOR (rat): S367‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

S263-p - DEPTOR (human)
Orthologous residues
DEPTOR (human): S263‑p, DEPTOR (mouse): S263‑p, DEPTOR (rat): S370‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mass spectrometry (in vitro), mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK1A (human)

S265-p - DEPTOR (human)
Orthologous residues
DEPTOR (human): S265‑p, DEPTOR (mouse): S265‑p, DEPTOR (rat): S372‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mass spectrometry (in vitro), mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE mTOR (human)

S267-p - DEPTOR (human)
Orthologous residues
DEPTOR (human): S267‑p, DEPTOR (mouse): S267‑p, DEPTOR (rat): S374‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

S280-p - DEPTOR (human)
Orthologous residues
DEPTOR (human): S280‑p, DEPTOR (mouse): S280‑p, DEPTOR (rat): S387‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

S283-p - DEPTOR (human)
Orthologous residues
DEPTOR (human): S283‑p, DEPTOR (mouse): S283‑p, DEPTOR (rat): S390‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

S286-p - DEPTOR (human)
Orthologous residues
DEPTOR (human): S286‑p, DEPTOR (mouse): S286‑p, DEPTOR (rat): S393‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mass spectrometry (in vitro), mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK1A (human)
KINASE mTOR (human)
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, protein degradation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
BTRC (human) Induces co-immunoprecipitation

S287-p - DEPTOR (human)
Orthologous residues
DEPTOR (human): S287‑p, DEPTOR (mouse): S287‑p, DEPTOR (rat): S394‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mass spectrometry (in vitro), mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK1A (human)

S293-p - DEPTOR (human)
Orthologous residues
DEPTOR (human): S293‑p, DEPTOR (mouse): S293‑p, DEPTOR (rat): S400‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mass spectrometry (in vitro), mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE mTOR (human)
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
BTRC (human) Induces co-immunoprecipitation

T295-p - DEPTOR (human)
Orthologous residues
DEPTOR (human): T295‑p, DEPTOR (mouse): T295‑p, DEPTOR (rat): T402‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mass spectrometry (in vitro), mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE mTOR (human)
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, protein degradation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
BTRC (human) Induces co-immunoprecipitation

S297-p - DEPTOR (human)
Orthologous residues
DEPTOR (human): S297‑p, DEPTOR (mouse): S297‑p, DEPTOR (rat): S404‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

S298-p - DEPTOR (human)
Orthologous residues
DEPTOR (human): S298‑p, DEPTOR (mouse): S298‑p, DEPTOR (rat): S405‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

S299-p - DEPTOR (human)
Orthologous residues
DEPTOR (human): S299‑p, DEPTOR (mouse): S299‑p, DEPTOR (rat): S406‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mass spectrometry (in vitro), mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE mTOR (human)
KINASE mTOR (human)
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, protein degradation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
BTRC (human) Induces co-immunoprecipitation


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