Curated Information
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PubMed Id: 22004453 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Doggett EA, et al. (2012) Phosphorylation of LRRK2 serines 955 and 973 is disrupted by Parkinson's disease mutations and LRRK2 pharmacological inhibition. J Neurochem 120, 37-45 22004453
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S860-p - LRRK2 (human)
Orthologous residues
LRRK2 (human): S860‑p, LRRK2 (mouse): S860‑p, LRRK2 (rat): S859‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S910-p - LRRK2 (human)
Orthologous residues
LRRK2 (human): S910‑p, LRRK2 (mouse): S910‑p, LRRK2 (rat): S909‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LRRK2-IN1 decrease
Downstream Regulation
 Effect of modification (function):  intracellular localization, molecular association, regulation, phosphorylation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 beta (human) Induces co-immunoprecipitation, far-Western
 Comments:  stimulates phosphorylation of S973

S935-p - LRRK2 (human)
Orthologous residues
LRRK2 (human): S935‑p, LRRK2 (mouse): S935‑p, LRRK2 (rat): S934‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LRRK2-IN1 decrease
Downstream Regulation
 Effect of modification (function):  intracellular localization, molecular association, regulation, phosphorylation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 beta (human) Induces co-immunoprecipitation, far-Western
 Comments:  stimulates phosphorylation of S973

S955-p - LRRK2 (human)
Orthologous residues
LRRK2 (human): S955‑p, LRRK2 (mouse): S955‑p, LRRK2 (rat): S954‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LRRK2-IN1 decrease

S973-p - LRRK2 (human)
Orthologous residues
LRRK2 (human): S973‑p, LRRK2 (mouse): S973‑p, LRRK2 (rat): S972‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LRRK2-IN1 decrease

S976-p - LRRK2 (human)
Orthologous residues
LRRK2 (human): S976‑p, LRRK2 (mouse): S976‑p, LRRK2 (rat): S975‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

T1491-p - LRRK2 (human)
Orthologous residues
LRRK2 (human): T1491‑p, LRRK2 (mouse): T1491‑p, LRRK2 (rat): T1490‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE LRRK2 (human)

T1503-p - LRRK2 (human)
Orthologous residues
LRRK2 (human): T1503‑p, LRRK2 (mouse): T1503‑p, LRRK2 (rat): T1502‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE LRRK2 (human)

T2031-p - LRRK2 (human)
Orthologous residues
LRRK2 (human): T2031‑p, LRRK2 (mouse): T2031‑p, LRRK2 (rat): T2030‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S910-p - LRRK2 (mouse)
Orthologous residues
LRRK2 (human): S910‑p, LRRK2 (mouse): S910‑p, LRRK2 (rat): S909‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LRRK2-IN1 decrease

S935-p - LRRK2 (mouse)
Orthologous residues
LRRK2 (human): S935‑p, LRRK2 (mouse): S935‑p, LRRK2 (rat): S934‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LRRK2-IN1 decrease

S955-p - LRRK2 (mouse)
Orthologous residues
LRRK2 (human): S955‑p, LRRK2 (mouse): S955‑p, LRRK2 (rat): S954‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LRRK2-IN1 decrease

S973-p - LRRK2 (mouse)
Orthologous residues
LRRK2 (human): S973‑p, LRRK2 (mouse): S973‑p, LRRK2 (rat): S972‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LRRK2-IN1 decrease


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