Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Curated Information Page
PubMed Id: 21892182 
Lee J, et al. (2011) p38 MAPK-mediated regulation of Xbp1s is crucial for glucose homeostasis. Nat Med 17, 1251-60 21892182
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

T48-p - XBP1 (mouse)
Orthologous residues
XBP1 (human): L55‑p, XBP1 iso2 (human): L55‑p, XBP1 (mouse): T48‑p, XBP1 iso2 (mouse): T48‑p, XBP1 (rat): T48‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), adipose tissue, liver, muscle
 Cellular systems studied:  cell lines, tissue
 Species studied:  human, mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE P38A (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE P38A (mouse) pharmacological inhibitor of upstream enzyme, activation of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anisomycin increase
fasting decrease in lean (wt) mice
refeeding increase in lean (wt ) mice
SB203580 refeeding inhibit treatment-induced increase in lean (wt) mice
refeeding decrease in obese (ob/ob) mice
Downstream Regulation
 Effect of modification (function):  intracellular localization
 Comments:  important for nuclear translocation of XBP1- not in obese (ob/ob) mice

S61-p - XBP1 (mouse)
Orthologous residues
XBP1 (human): S68‑p, XBP1 iso2 (human): S68‑p, XBP1 (mouse): S61‑p, XBP1 iso2 (mouse): S61‑p, XBP1 (rat): S61‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), adipose tissue, liver, MEF (fibroblast), muscle
 Cellular systems studied:  cell lines, tissue
 Species studied:  human, mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE P38A (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE P38A (mouse) pharmacological inhibitor of upstream enzyme, activation of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anisomycin increase
fasting decrease in lean (wt) mice
refeeding increase in lean (wt ) mice
SB203580 refeeding inhibit treatment-induced increase in lean (wt) mice
refeeding decrease in obese (ob/ob) mice
Downstream Regulation
 Effect of modification (function):  intracellular localization
 Comments:  important for nuclear translocation of XBP1- not in obese (ob/ob) mice


Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.