Curated Information
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Curated Information Page
PubMed Id: 21969604 
Rowland AF, Larance M, Hughes WE, James DE (2011) Identification of RhoGAP22 as an Akt-Dependent Regulator of Cell Motility in Response to Insulin. Mol Cell Biol 31, 4789-800 21969604
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S16-p - ARHGAP22 (human)
Orthologous residues
ARHGAP22 (human): S16‑p, ARHGAP22 iso5 (human): S22‑p, ARHGAP22 (mouse): S22‑p, ARHGAP22 (rat):
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  CHO (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  hamster
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
wortmannin insulin inhibit treatment-induced increase
Akt-I-1 insulin inhibit treatment-induced increase
rapamycin insulin no effect upon treatment-induced increase
Y27632 insulin no effect upon treatment-induced increase
PD98059 insulin no effect upon treatment-induced increase
Go 6983 insulin no effect upon treatment-induced increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell motility, induced
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 beta (human) Induces far-Western, co-immunoprecipitation
 Comments:  binding of 14-3-3 beta to ARHGAP22 S395 depends on its binding to S16

S359-p - ARHGAP22 (human)
Orthologous residues
ARHGAP22 (human): S359‑p, ARHGAP22 iso5 (human): S365‑p, ARHGAP22 (mouse): S365‑p, ARHGAP22 (rat): S200‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  CHO (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  hamster

S395-p - ARHGAP22 (human)
Orthologous residues
ARHGAP22 (human): S395‑p, ARHGAP22 iso5 (human): S401‑p, ARHGAP22 (mouse): S397‑p, ARHGAP22 (rat): S236‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  CHO (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  hamster
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
wortmannin insulin inhibit treatment-induced increase
Akt-I-1 insulin inhibit treatment-induced increase
rapamycin insulin no effect upon treatment-induced increase
Y27632 insulin no effect upon treatment-induced increase
PD98059 insulin no effect upon treatment-induced increase
Go 6983 insulin no effect upon treatment-induced increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell motility, induced
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 beta (human) Induces far-Western, co-immunoprecipitation
 Comments:  binding of 14-3-3 beta to ARHGAP22 S395 depends on its binding to S16

S476-p - ARHGAP22 (human)
Orthologous residues
ARHGAP22 (human): S476‑p, ARHGAP22 iso5 (human): S482‑p, ARHGAP22 (mouse): S483‑p, ARHGAP22 (rat): S322‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  CHO (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  hamster


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