Curated Information
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Curated Information Page
PubMed Id: 12769847 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Cory GO, Cramer R, Blanchoin L, Ridley AJ (2003) Phosphorylation of the WASP-VCA domain increases its affinity for the Arp2/3 complex and enhances actin polymerization by WASP. Mol Cell 11, 1229-39 12769847
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S483-p - WASP (human)
Orthologous residues
WASP (human): S483‑p, WASP (mouse): S501‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  BAC1.2F5 (macrophage), COS (fibroblast), Jurkat (T lymphocyte), Raji (B lymphocyte), Swiss 3T3 (fibroblast), U-937 (myeloid)
 Cellular systems studied:  cell lines
 Species studied:  human, monkey, mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK2A1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LY294002 decrease
DRB decrease
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cytoskeletal reorganization
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
ARPC3 (human) Induces not reported co-immunoprecipitation
 Comments:  The double mutant (S483A/S484A) was used in this study.

S484-p - WASP (human)
Orthologous residues
WASP (human): S484‑p, WASP (mouse): S502‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  BAC1.2F5 (macrophage), COS (fibroblast), Jurkat (T lymphocyte), Raji (B lymphocyte), Swiss 3T3 (fibroblast), U-937 (myeloid)
 Cellular systems studied:  cell lines, primary cells
 Species studied:  human, monkey, mouse
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK2A1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DRB decrease
LY294002 decrease
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cytoskeletal reorganization
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
ARPC3 (human) Induces not reported co-immunoprecipitation
 Comments:  The double mutant (S483A/S484A) was used in this study.


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