Curated Information
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Curated Information Page
PubMed Id: 21571219 
Zhou P, et al. (2011) Numb links extracellular cues to intracellular polarity machinery to promote chemotaxis. Dev Cell 20, 610-22 21571219
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T410-p - PKCZ (human)
Orthologous residues
PKCZ (human): T410‑p, PKCZ (mouse): T410‑p, PKCZ (rat): T410‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BDNF increase
K252a BDNF inhibit treatment-induced increase
BDNF NUMB (mouse) increase NUMB -/- inhibits

Y496-p - TrkA (human)
Orthologous residues
TrkA (human): Y496‑p, TrkA iso2 (human): Y490‑p, TrkA (mouse): Y499‑p, TrkA (rat): Y499‑p, TrkA iso2 (rat): Y493‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BDNF increase
K252a BDNF inhibit treatment-induced increase

S295-p - NUMB (mouse)
Orthologous residues
NUMB (human): S295‑p, NUMB iso2 (human): S295‑p, NUMB iso3 (human): S284‑p, NUMB (mouse): S295‑p, NUMB iso2 (mouse): S295‑p, NUMB (rat): S294‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCZ (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCZ (mouse) mutation in upstream enzyme recognition motif, microscopy-colocalization, pharmacological inhibitor of upstream enzyme, activation of upstream enzyme
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell motility, induced, endocytosis, induced
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
TrkA (rat) Induces endocytosis, induced, cell motility, induced co-immunoprecipitation


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