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Orthologous residues
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ataxin‑1 (human): S775‑p, ataxin‑1 (mouse): S751‑p, ataxin‑1 (rat): S749‑p
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Characterization
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Methods used to characterize site in vivo:
immunoprecipitation, mutation of modification site, western blotting
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Relevant cell lines - cell types - tissues:
COS (fibroblast), HeLa (cervical)
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|
Cellular systems studied:
cell lines
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Species studied:
human, monkey
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Enzymes shown to modify site in vitro:
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|
|
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Upstream Regulation
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|
Potential in vivo enzymes for site:
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Type
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Enzyme
|
Evidence
|
Notes
|
|
KINASE
|
Akt1 (human)
|
transfection of constitutively active enzyme, transfection of dominant-negative enzyme
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|
|
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Downstream Regulation
|
|
Effect of modification (function):
molecular association, regulation, protein stabilization
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|
Modification regulates interactions with:
|
|
Interacting molecule
|
Interacting domains
|
Effect
|
Consequences (function)
|
Consequences (process)
|
Detection assays
|
|
14-3-3 epsilon (human)
|
|
Induces
|
molecular association, regulation
|
|
co-immunoprecipitation, in vitro
|
|
14-3-3 epsilon (human)
|
|
Induces
|
protein stabilization
|
cytoskeletal reorganization
|
co-immunoprecipitation, yeast two-hybrid, electrophoretic visualization
|
|
14-3-3 beta (human)
|
|
Induces
|
protein stabilization
|
cytoskeletal reorganization
|
co-immunoprecipitation, yeast two-hybrid, electrophoretic visualization
|
|
14-3-3 zeta (human)
|
|
Induces
|
protein stabilization
|
cytoskeletal reorganization
|
co-immunoprecipitation, yeast two-hybrid, electrophoretic visualization
|
|
|
Comments:
Inclusion bodies form in the nucleus. Stabilization of ataxin-1 and neurodegeneration require PI3K/Akt signaling in flies.; Interaction requires S776 phosphorylation.
|
