Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Curated Information Page
PubMed Id: 12757707 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Chen HK, et al. (2003) Interaction of Akt-phosphorylated ataxin-1 with 14-3-3 mediates neurodegeneration in spinocerebellar ataxia type 1. Cell 113, 457-68 12757707
Download Sites

S775-p - ataxin-1 (human)
Orthologous residues
ataxin‑1 (human): S775‑p, ataxin‑1 (mouse): S751‑p, ataxin‑1 (rat): S749‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human, monkey
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (human) transfection of constitutively active enzyme, transfection of dominant-negative enzyme
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, protein stabilization
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 epsilon (human) Induces protein stabilization cytoskeletal reorganization electrophoretic visualization, yeast two-hybrid, co-immunoprecipitation
14-3-3 epsilon (human) Induces molecular association, regulation in vitro, co-immunoprecipitation
14-3-3 beta (human) Induces protein stabilization cytoskeletal reorganization electrophoretic visualization, yeast two-hybrid, co-immunoprecipitation
14-3-3 zeta (human) Induces protein stabilization cytoskeletal reorganization electrophoretic visualization, yeast two-hybrid, co-immunoprecipitation
 Comments:  Inclusion bodies form in the nucleus. Stabilization of ataxin-1 and neurodegeneration require PI3K/Akt signaling in flies.; Interaction requires S776 phosphorylation.


Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.