Wen H, et al. (2011) Fatty acid-induced NLRP3-ASC inflammasome activation interferes with insulin signaling. Nat Immunol 12, 408-15 21478880

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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S79-p - ACC1 (mouse) ▲

Modsite: FHMRSsMsGLHLVKQ SwissProt Entrez-Gene Orthologous residues ACC1 (human): S80‑p, ACC1 iso2 (human): S22‑p, ACC1 iso4 (human): S117‑p, ACC1 (mouse): S79‑p, ACC1 iso2 (mouse): S117‑p, ACC1 (rat): S79‑p, ACC1 iso2 (rat): S79‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  macrophage-bone marrow Cellular systems studied:  primary cells Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes acadesine increase

S473-p - Akt1 (mouse) ▲

Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene Orthologous residues Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  leukemia Relevant cell lines - cell types - tissues:  adipose tissue, FL, liver, muscle Cellular systems studied:  cell lines, primary cells Species studied:  mouse Comments:  FL83B cell line Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes insulin increase IL-1b insulin inhibit treatment-induced increase TNF insulin inhibit treatment-induced increase insulin NLRP3 (mouse) inhibit treatment-induced increase NLRP3 depletion increases insulin PYCARD (mouse) inhibit treatment-induced increase PYCARD depletion increases CASP1 (mouse) inhibit treatment-induced increase Caspase-1 depletion increases antibody increase

T183-p - AMPKA1 (mouse) ▲

Modsite: sDGEFLRtsCGsPNY SwissProt Entrez-Gene Orthologous residues AMPKA1 (human): T183‑p, AMPKA1 iso2 (human): T198‑p, AMPKA1 (mouse): T183‑p, AMPKA1 (rat): T183‑p, AMPKA1 (fruit fly): T184‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  macrophage-bone marrow Cellular systems studied:  primary cells Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes LPS, palmitate decrease palmitate no change compared to control LPS no change compared to control acadesine LPS, palmitate inhibit treatment-induced decrease Downstream Regulation Effect of modification (function):  enzymatic activity, induced Effect of modification (process):  autophagy, induced Comments:  reduced ROS generation and increased autophagosomes

S307-p - IRS1 (mouse) ▲

Modsite: tEsITAtsPAsMVGG SwissProt Entrez-Gene Orthologous residues IRS1 (human): S312‑p, IRS1 (mouse): S307‑p, IRS1 (rat): S307‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  leukemia Relevant cell lines - cell types - tissues:  adipose tissue, FL, liver, muscle Cellular systems studied:  cell lines, primary cells Species studied:  mouse Comments:  FL83B cell line Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes TNF increase IL-1b increase insulin increase

S467-p - ULK1 (mouse) ▲
Modsite: sAIRRsGsttPLGFG SwissProt Entrez-Gene Orthologous residues ULK1 (human): S467‑p, ULK1 (mouse): S467‑p, ULK1 (rat): S467‑p Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes acadesine increase

S555-p - ULK1 (mouse) ▲
Modsite: GLGCRLHsAPNLSDF SwissProt Entrez-Gene Orthologous residues ULK1 (human): S556‑p, ULK1 (mouse): S555‑p, ULK1 (rat): S555‑p Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes acadesine increase LPS increase palmitate LPS inhibit treatment-induced increase