Curated Information
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Curated Information Page
PubMed Id: 12591950 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Lei K, Davis RJ (2003) JNK phosphorylation of Bim-related members of the Bcl2 family induces Bax-dependent apoptosis. Proc Natl Acad Sci U S A 100, 2432-7 12591950
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S44-p - Bim iso2 (human)
Orthologous residues
Bim (human): S104‑p, Bim iso2 (human): S44‑p, Bim iso3 (human): , Bim (mouse): S100‑p, Bim (rat): S100‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site, phosphoamino acid analysis
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE JNK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JNK1 (human) transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase

T56-p - Bim iso2 (human)
Orthologous residues
Bim (human): T116‑p, Bim iso2 (human): T56‑p, Bim iso3 (human): , Bim (mouse): T112‑p, Bim (rat): T112‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site, phosphoamino acid analysis
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE JNK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JNK1 (human) transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
Downstream Regulation
 Effect of modification (function):  activity, induced, molecular association, regulation
 Effect of modification (process):  apoptosis, induced
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
LC8 (human) Disrupts co-immunoprecipitation, electrophoretic visualization

S58-p - Bim iso2 (human)
Orthologous residues
Bim (human): S118‑p, Bim iso2 (human): S58‑p, Bim iso3 (human): , Bim (mouse): S114‑p, Bim (rat): S114‑p
Characterization
 Methods used to characterize site in vivo [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site, phosphoamino acid analysis
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE JNK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JNK1 (human) transfection of inactive enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase

S74-p - BMF (human)
Orthologous residues
BMF (human): S74‑p, BMF (mouse): S74‑p
Characterization
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE JNK1 (human)


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