Curated Information
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Curated Information Page
PubMed Id: 21878642 
Xiao L, et al. (2011) KIBRA Protein Phosphorylation Is Regulated by Mitotic Kinase Aurora and Protein Phosphatase 1. J Biol Chem 286, 36304-15 21878642
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
Download Sites

T308-p - Akt1 (human)
Orthologous residues
Akt1 (human): T308‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
okadaic acid increase (50 nM inhibits PP2A)

T288-p - AurA (human)
Orthologous residues
AurA (human): T288‑p, AurA (mouse): T279‑p, AurA (rat): T281‑p, AurA (pig): T288‑p, AurA (frog): T295‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase nocodazole arrested cells
VX-680 decrease

T232-p - AurB (human)
Orthologous residues
AurB (human): T232‑p, AurB (mouse): T237‑p, AurB (rat): T235‑p, AurB (pig): T232‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase nocodazole arrested cells
VX-680 decrease

Y15-p - CDK1 (human)
Orthologous residues
CDK1 (human): Y15‑p, CDK1 (mouse): Y15‑p, CDK1 (rat): Y15‑p, CDK1 (chicken): Y15‑p, CDK1 (fruit fly): Y15‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

S11-p - H3 (human)
Orthologous residues
H3 (human): S11‑p, H3 (mouse): S11‑p, H3 iso2 (mouse): S11‑p, H3 iso3 (mouse): S11‑p, H3 (rat): S11‑p, H3 iso3 (rat): S11‑p, H3 (pig): S11‑p, H3 (chicken): S11‑p, H3 (cow): S11‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
thymidine increase

S539-p - KIBRA (human)
Orthologous residues
KIBRA (human): S539‑p, KIBRA iso2 (human): S539‑p, KIBRA (mouse): S539‑p, KIBRA (rat): S539‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurB (human)
PHOSPHATASE PPP1CA (human)
KINASE AurA (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE AurB (human) modification site within consensus motif, transfection of inactive enzyme, mutation in upstream enzyme recognition motif, transfection of wild-type enzyme, siRNA inhibition of enzyme, phospho-antibody
KINASE AurA (human) modification site within consensus motif, transfection of inactive enzyme, co-immunoprecipitation, mutation in upstream enzyme recognition motif, transfection of wild-type enzyme, siRNA inhibition of enzyme, phospho-antibody
PHOSPHATASE PPP1CA (human) transfection of dominant-negative enzyme, co-immunoprecipitation, transfection of wild-type enzyme, pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme, phospho-antibody
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Effect of modification (process):  cell cycle regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Merlin (human) Induces co-immunoprecipitation

S127-p - YAP1 (human)
Orthologous residues
YAP1 (human): S127‑p, YAP1 iso2 (human): S127‑p, YAP1 iso3 (human): S127‑p, YAP1 (mouse): S112‑p, YAP1 (rat): S112‑p, YAP1 iso3 (rat): S112‑p, YAP1 (sheep): S42‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human


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