Curated Information
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PubMed Id: 21765415 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Shembade N, et al. (2011) The kinase IKKα inhibits activation of the transcription factor NF-κB by phosphorylating the regulatory molecule TAX1BP1. Nat Immunol 12, 834-43 21765415
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S593-p - TAX1BP1 (human) ▲
Orthologous residues
TAX1BP1 (human): S593‑p, TAX1BP1 iso2 (human): S593‑p, TAX1BP1 (mouse): S619‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S666-p - TAX1BP1 (human) ▲
Orthologous residues
TAX1BP1 (human): S666‑p, TAX1BP1 iso2 (human): S624‑p, TAX1BP1 (mouse): S693‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S593-p - TAX1BP1 iso2 (human) ▲
Orthologous residues
TAX1BP1 (human): S593‑p, TAX1BP1 iso2 (human): S593‑p, TAX1BP1 (mouse): S619‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), macrophage-bone marrow, MEF (fibroblast)
 Cellular systems studied:  cell lines, primary cultured cells
 Species studied:  human, mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE IKK-alpha (human) modification site within consensus motif, siRNA inhibition of enzyme, transfection of wild-type enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
IL-1a increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, ubiquitination
 Effect of modification (process):  signaling pathway regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
TRAF2 (human) Induces co-immunoprecipitation
TRAF6 (human) Induces co-immunoprecipitation
 Comments:  regulates NF-kB signaling; regulates interaction with A20 and Itch via TRAF2 and TRAF6;

S624-p - TAX1BP1 iso2 (human) ▲
Orthologous residues
TAX1BP1 (human): S666‑p, TAX1BP1 iso2 (human): S624‑p, TAX1BP1 (mouse): S693‑p
Characterization
 Methods used to characterize site in vivo:  electrophoretic mobility shift, mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  293T (epithelial), macrophage-bone marrow, MEF (fibroblast)
 Cellular systems studied:  cell lines, primary cultured cells
 Species studied:  human, mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE IKK-alpha (human) electrophoretic mobility shift, siRNA inhibition of enzyme, transfection of wild-type enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
IL-1a increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, ubiquitination
 Effect of modification (process):  signaling pathway regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
TRAF2 (human) Induces co-immunoprecipitation
TRAF6 (human) Induces co-immunoprecipitation
 Comments:  regulates NF-kB signaling; regulates interaction with A20 and Itch via TRAF2 and TRAF6;

S619-p - TAX1BP1 (mouse) ▲
Orthologous residues
TAX1BP1 (human): S593‑p, TAX1BP1 iso2 (human): S593‑p, TAX1BP1 (mouse): S619‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  macrophage-bone marrow
 Cellular systems studied:  primary cultured cells
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
IL-1a increase


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