Curated Information
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Curated Information Page
PubMed Id: 12588975 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Wang X, et al. (2003) The C terminus of initiation factor 4E-binding protein 1 contains multiple regulatory features that influence its function and phosphorylation. Mol Cell Biol 23, 1546-57 12588975
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T37-p - 4E-BP1 (human) ▲
Orthologous residues
4E‑BP1 (human): T37‑p, 4E‑BP1 (mouse): T36‑p, 4E‑BP1 (rat): T36‑p, 4E‑BP1 (fruit fly): T37‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  phosphorylation
 Comments:  Deletion of (QFEMDI) sequence affected phosphorlation of multiple phosphorylation sites.

T46-p - 4E-BP1 (human) ▲
Orthologous residues
4E‑BP1 (human): T46‑p, 4E‑BP1 (mouse): T45‑p, 4E‑BP1 (rat): T45‑p, 4E‑BP1 (fruit fly): T46‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  phosphorylation
 Comments:  Deletion of (QFEMDI) sequence affected phosphorlation of multiple phosphorylation sites.

S65-p - 4E-BP1 (human) ▲
Orthologous residues
4E‑BP1 (human): S65‑p, 4E‑BP1 (mouse): S64‑p, 4E‑BP1 (rat): S64‑p, 4E‑BP1 (fruit fly): S65‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE DYRK2 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
rapamycin no change compared to control
LY294002 no change compared to control
SB202190 no change compared to control
PD184352 no change compared to control

T70-p - 4E-BP1 (human) ▲
Orthologous residues
4E‑BP1 (human): T70‑p, 4E‑BP1 (mouse): T69‑p, 4E‑BP1 (rat): T69‑p, 4E‑BP1 (fruit fly): T70‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  phosphorylation
 Comments:  Deletion of (QFEMDI) sequence affected phosphorlation of multiple phosphorylation sites.

S101-p - 4E-BP1 (human) ▲
Orthologous residues
4E‑BP1 (human): S101‑p, 4E‑BP1 (mouse): S100‑p, 4E‑BP1 (rat): S100‑p, 4E‑BP1 (fruit fly): ‑
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE DYRK2 (human)

S112-p - 4E-BP1 (human) ▲
Orthologous residues
4E‑BP1 (human): S112‑p, 4E‑BP1 (mouse): S111‑p, 4E‑BP1 (rat): S111‑p, 4E‑BP1 (fruit fly): ‑
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphopeptide mapping
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE CK2A1 (human)
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
rapamycin no change compared to control
wortmannin no change compared to control
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, phosphorylation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
eIF4E (mouse) Disrupts co-immunoprecipitation, electrophoretic visualization
 Comments:  Deletion of (QFEMDI) sequence affected phosphorlation of multiple phosphorylation sites.; S111 did not affect binding to EIF4E.


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