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Orthologous residues
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TK (human): S13‑p, TK (mouse): S13‑p
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Characterization
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Methods used to characterize site in vivo:
[32P] bio-synthetic labeling, mutation of modification site
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Relevant cell lines - cell types - tissues:
HeLa (cervical) [TK (human)]
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Cellular systems studied:
cell lines
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Species studied:
human, mouse
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Enzymes shown to modify site in vitro:
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Comments:
The authors used an extract containing both Cdc2 and Cdk2, so it is not clear if one or both kinases are active against this site.
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Upstream Regulation
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Treatments, proteins and their effect on site modification:
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Treatments
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Referenced Treatments
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Manipulated Protein
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Referenced Protein
|
Effect
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Notes
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nocodazole
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increase
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