|
Orthologous residues
|
|
H2AX (human): S140‑p, H2AX (mouse): S140‑p, H2AX (rat): S140‑p
|
|
Characterization
|
|
Methods used to characterize site in vivo:
phospho-antibody
|
|
Relevant cell lines - cell types - tissues:
MEF (fibroblast)
|
|
Cellular systems studied:
primary cultured cells
|
|
Species studied:
mouse
|
|
Enzymes shown to modify site in vitro:
|
|
|
|
Upstream Regulation
|
|
Potential in vivo enzymes for site:
|
|
Type
|
Enzyme
|
Evidence
|
Notes
|
|
KINASE
|
ATM (mouse)
|
genetic knockout/knockin of upstream enzyme, genetic transfer of wild-type enzyme
|
|
|
|
Treatments, proteins and their effect on site modification:
|
|
Treatments
|
Referenced Treatments
|
Manipulated Protein
|
Referenced Protein
|
Effect
|
Notes
|
|
neocarzinostatin
|
|
|
|
increase
|
|
|
bleomycin
|
|
|
|
increase
|
|
|
ionizing radiation
|
|
|
|
increase
|
|
|
wortmannin
|
|
|
|
decrease
|
|
|
etoposide
|
|
|
|
increase
|
|
|
|
Downstream Regulation
|
|
Effect of modification (function):
activation
|
