Curated Information
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Curated Information Page
PubMed Id: 11914378 
Saitoh M, et al. (2002) Regulation of an activated S6 kinase 1 variant reveals a novel mammalian target of rapamycin phosphorylation site. J Biol Chem 277, 20104-12 11914378
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T252-p - p70S6K (human)
Orthologous residues
p70S6K (human): T252‑p, p70S6K iso2 (human): T229‑p, p70S6K (mouse): T252‑p, p70S6K (rat): T252‑p, p70S6K iso2 (rat): T229‑p, p70S6K (fruit fly): T238‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
wortmannin insulin inhibit treatment-induced increase
rapamycin insulin inhibit treatment-induced increase

S394-p - p70S6K (human)
Orthologous residues
p70S6K (human): S394‑p, p70S6K iso2 (human): S371‑p, p70S6K (mouse): S394‑p, p70S6K (rat): S394‑p, p70S6K iso2 (rat): S371‑p, p70S6K (fruit fly): S380‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE mTOR (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE mTOR (human) phospho-antibody, genetic transfer of dominant-negative enzyme, genetic transfer of wild-type enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
wortmannin insulin inhibit treatment-induced increase
rapamycin insulin inhibit treatment-induced increase
serum increase
rapamycin serum inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  activity, induced

T412-p - p70S6K (human)
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p


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