Curated Information
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Curated Information Page
PubMed Id: 18215134 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Zhou QL, et al. (2008) Akt substrate TBC1D1 regulates GLUT1 expression through the mTOR pathway in 3T3-L1 adipocytes. Biochem J 411, 647-55 18215134
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T412-p - p70S6K (mouse) ▲
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast), CHO (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  hamster, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
siRNA TBC1D1 (mouse) increase
insulin increase
siRNA insulin TBC1D1 (mouse) no effect upon treatment-induced increase

S235-p - S6 (mouse) ▲
Orthologous residues
S6 (human): S235‑p, S6 (mouse): S235‑p, S6 (rat): S235‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast), CHO (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  hamster, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
siRNA TBC1D1 (mouse) increase
insulin increase
siRNA insulin TBC1D1 (mouse) no effect upon treatment-induced increase
rapamycin decrease

S236-p - S6 (mouse) ▲
Orthologous residues
S6 (human): S236‑p, S6 (mouse): S236‑p, S6 (rat): S236‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast), CHO (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  hamster, mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
siRNA TBC1D1 (mouse) increase
insulin increase
siRNA insulin TBC1D1 (mouse) no effect upon treatment-induced increase
rapamycin decrease

T590-p - TBC1D1 (mouse) ▲
Orthologous residues
TBC1D1 (human): T596‑p, TBC1D1 (mouse): T590‑p, TBC1D1 iso2 (mouse): T590‑p, TBC1D1 (rat): T590‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast), CHO (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  hamster, mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (mouse) pharmacological activator of upstream enzyme, phospho-motif antibody, modification site within consensus motif
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
Downstream Regulation
 Effect of modification (function):  phosphorylation
 Comments:  p70S6K phosphorylation


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