Curated Information
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PubMed Id: 18303026 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Tatematsu K, et al. (2008) Identification of ubiquitin ligase activity of RBCK1 and its inhibition by splice variant RBCK2 and protein kinase Cbeta. J Biol Chem 283, 11575-85 18303026
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S137-p - RBCK1 (rat)
Orthologous residues
RBCK1 (human): S137‑p, RBCK1 (mouse): S137‑p, RBCK1 (rat): S137‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial), 293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  phosphorylation of S137, T161, T207, S270, T275 and/or S285 identified by alanine mutation of all six sites
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCB (human)

T161-p - RBCK1 (rat)
Orthologous residues
RBCK1 (human): T161‑p, RBCK1 (mouse): T161‑p, RBCK1 (rat): T161‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial), 293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  phosphorylation of S137, T161, T207, S270, T275 and/or S285 identified by alanine mutation of all six sites
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCB (human)
Downstream Regulation
 Effect of modification (function):  protein stabilization
 Comments:  inhibits self-ubiquitination of RBCK1 in vitro

T207-p - RBCK1 (rat)
Orthologous residues
RBCK1 (human): T209‑p, RBCK1 (mouse): T207‑p, RBCK1 (rat): T207‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial), 293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  phosphorylation of S137, T161, T207, S270, T275 and/or S285 identified by alanine mutation of all six sites
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCB (human)
Downstream Regulation
 Effect of modification (function):  protein stabilization
 Comments:  inhibits self-ubiquitination of RBCK1 in vitro

S270-p - RBCK1 (rat)
Orthologous residues
RBCK1 (human): S272‑p, RBCK1 (mouse): S270‑p, RBCK1 (rat): S270‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial), 293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  phosphorylation of S137, T161, T207, S270, T275 and/or S285 identified by alanine mutation of all six sites
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCB (human)

T275-p - RBCK1 (rat)
Orthologous residues
RBCK1 (human): T277‑p, RBCK1 (mouse): T275‑p, RBCK1 (rat): T275‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial), 293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  phosphorylation of S137, T161, T207, S270, T275 and/or S285 identified by alanine mutation of all six sites
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCB (human)

S285-p - RBCK1 (rat)
Orthologous residues
RBCK1 (human): S287‑p, RBCK1 (mouse): S285‑p, RBCK1 (rat): S285‑p
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial), 293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Comments:  phosphorylation of S137, T161, T207, S270, T275 and/or S285 identified by alanine mutation of all six sites
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCB (human)


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