Curated Information
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PubMed Id: 16407412 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Balan V, et al. (2006) Identification of novel in vivo Raf-1 phosphorylation sites mediating positive feedback Raf-1 regulation by extracellular signal-regulated kinase. Mol Biol Cell 17, 1141-53 16407412
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S29-p - c-Raf (human) ▲
Orthologous residues
c‑Raf (human): S29‑p, c‑Raf (mouse): S29‑p, c‑Raf (rat): S29‑p, c‑Raf (chicken): S29‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey

S43-p - c-Raf (human) ▲
Orthologous residues
c‑Raf (human): S43‑p, c‑Raf (mouse): S43‑p, c‑Raf (rat): S43‑p, c‑Raf (chicken): S43‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey

S259-p - c-Raf (human) ▲
Orthologous residues
c‑Raf (human): S259‑p, c‑Raf (mouse): S259‑p, c‑Raf (rat): S259‑p, c‑Raf (chicken): S259‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey

S289-p - c-Raf (human) ▲
Orthologous residues
c‑Raf (human): S289‑p, c‑Raf (mouse): S289‑p, c‑Raf (rat): S289‑p, c‑Raf (chicken): S289‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ERK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK1 (human) phospho-antibody, pharmacological inhibitor of upstream enzyme, transfection of wild-type enzyme, pharmacological activator of upstream enzyme, activation of upstream enzyme
 Comments:  phosphoantibody is for S296 only.
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase
EGF ERK1 (human) augment treatment-induced increase
U0126 EGF inhibit treatment-induced increase
siRNA inhibit treatment-induced increase Erk1/2 siRNA
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced
 Comments:  phosphorylation of S289, S296, and S301 sustains and increases kinase activity

S296-p - c-Raf (human) ▲
Orthologous residues
c‑Raf (human): S296‑p, c‑Raf (mouse): S296‑p, c‑Raf (rat): S296‑p, c‑Raf (chicken): S296‑p
Characterization
 Methods used to characterize site in vivo:  2D analysis, [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site, phospho-antibody, phosphoamino acid analysis, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ERK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK1 (human) phospho-antibody, pharmacological inhibitor of upstream enzyme, transfection of wild-type enzyme, pharmacological activator of upstream enzyme, activation of upstream enzyme
 Comments:  phosphoantibody is for S296 only.
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase
EGF ERK1 (human) augment treatment-induced increase
U0126 EGF inhibit treatment-induced increase
siRNA inhibit treatment-induced increase Erk1/2 siRNA
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced
 Comments:  phosphorylation of S289, S296, and S301 sustains and increases kinase activity

S301-p - c-Raf (human) ▲
Orthologous residues
c‑Raf (human): S301‑p, c‑Raf (mouse): S301‑p, c‑Raf (rat): S301‑p, c‑Raf (chicken): S301‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE ERK1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK1 (human) phospho-antibody, pharmacological inhibitor of upstream enzyme, transfection of wild-type enzyme, pharmacological activator of upstream enzyme, activation of upstream enzyme
 Comments:  phosphoantibody is for S296 only.
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase
EGF ERK1 (human) augment treatment-induced increase
U0126 EGF inhibit treatment-induced increase
siRNA inhibit treatment-induced increase Erk1/2 siRNA
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced
 Comments:  phosphorylation of S289, S296, and S301 sustains and increases kinase activity

S471-p - c-Raf (human) ▲
Orthologous residues
c‑Raf (human): S471‑p, c‑Raf (mouse): S471‑p, c‑Raf (rat): S471‑p, c‑Raf (chicken): S471‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey

T481-p - c-Raf (human) ▲
Orthologous residues
c‑Raf (human): T481‑p, c‑Raf (mouse): T481‑p, c‑Raf (rat): T481‑p, c‑Raf (chicken): T481‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey

S642-p - c-Raf (human) ▲
Orthologous residues
c‑Raf (human): S642‑p, c‑Raf (mouse): S642‑p, c‑Raf (rat): S642‑p, c‑Raf (chicken): T642‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey


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