|
Orthologous residues
|
|
ATM (human): S1981‑p, ATM (mouse): S1987‑p
|
|
Characterization
|
|
Methods used to characterize site in vivo:
2D analysis, [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phospho-antibody, phosphoamino acid analysis, western blotting
|
|
Relevant cell lines - cell types - tissues:
293T (epithelial), fibroblast-foreskin, HeLa (cervical)
|
|
Cellular systems studied:
cell lines, primary cultured cells
|
|
Species studied:
human
|
|
Enzymes shown to modify site in vitro:
|
|
|
|
Upstream Regulation
|
|
Treatments, proteins and their effect on site modification:
|
|
Treatments
|
Referenced Treatments
|
Manipulated Protein
|
Referenced Protein
|
Effect
|
Notes
|
|
ionizing radiation
|
|
|
|
increase
|
|
|
wortmannin
|
ionizing radiation
|
|
|
inhibit treatment-induced increase
|
|
|
UV
|
|
|
|
increase
|
|
|
chloroquine
|
|
|
|
increase
|
|
|
trichostatin A
|
|
|
|
increase
|
|
|
hypotonic buffer
|
|
|
|
increase
|
|
|
|
Downstream Regulation
|
|
Effect of modification (function):
intracellular localization, molecular association, regulation
|
|
Effect of modification (process):
cell cycle regulation
|
|
Modification regulates interactions with:
|
|
Interacting molecule
|
Interacting domains
|
Effect
|
Consequences (function)
|
Consequences (process)
|
Detection assays
|
|
p53 (human)
|
|
Induces
|
phosphorylation
|
|
functional assay, in vitro
|
|
