Curated Information
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Curated Information Page
PubMed Id: 12042314 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Fujita N, Sato S, Katayama K, Tsuruo T (2002) Akt-dependent phosphorylation of p27Kip1 promotes binding to 14-3-3 and cytoplasmic localization. J Biol Chem 277, 28706-13 12042314
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S58-p - 14-3-3 zeta (human) ▲
Orthologous residues
14‑3‑3 zeta (human): S58‑p, 14‑3‑3 zeta (mouse): S58‑p, 14‑3‑3 zeta (rat): S58‑p, 14‑3‑3 zeta (sheep): S58‑p

S10-p - p27Kip1 (human) ▲
Orthologous residues
p27Kip1 (human): S10‑p, p27Kip1 (mouse): S10‑p, p27Kip1 (rat): S10‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  293T (epithelial) [Akt1 (human)], 293T (epithelial) [p27Kip1 (human)]
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Akt1 (human)

T187-p - p27Kip1 (human) ▲
Orthologous residues
p27Kip1 (human): T187‑p, p27Kip1 (mouse): T187‑p, p27Kip1 (rat): T187‑p
Characterization
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE CDK2 (human)

T198-p - p27Kip1 (human) ▲
Orthologous residues
p27Kip1 (human): T198‑p, p27Kip1 (mouse): T197‑p, p27Kip1 (rat): T197‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody
 Relevant cell lines - cell types - tissues:  293T (epithelial) [Akt1 (human)], 293T (epithelial) [p27Kip1 (human)]
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE Akt1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (human)
Downstream Regulation
 Effect of modification (function):  intracellular localization, molecular association, regulation
 Effect of modification (process):  cell cycle regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 theta (human) 14-3-3 Induces intracellular localization co-immunoprecipitation
14-3-3 eta (human) 14-3-3 Induces intracellular localization co-immunoprecipitation
14-3-3 epsilon (human) 14-3-3 Induces intracellular localization co-immunoprecipitation
 Comments:  14-3-3-theta binds strongly to phospho-T198 in the co-precipitation assay, while -epsilon and -eta bind weakly.


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