Curated Information

Javascript is not enabled on this browser. This site will not work properly without Javascript.

Home

Curated Information Page

PubMed Id: 16418171

This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus^®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus^®, click here.

Information from this record has been curated, but not yet edited in PhosphoSitePlus^® and may be incomplete.

Weigert C, et al. (2006) Direct cross-talk of interleukin-6 and insulin signal transduction via insulin receptor substrate-1 in skeletal muscle cells. J Biol Chem 281, 7060-7 16418171

Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.

S307-p - IRS1 (mouse)

▲

Orthologous residues

IRS1 (human): S312‑p, IRS1 (mouse): S307‑p, IRS1 (rat): S307‑p

Characterization

Methods used to characterize site in vivo: phospho-antibody, western blotting

Relevant cell lines - cell types - tissues: 'muscle, skeletal', C2C12 (myoblast), L6 (myoblast), liver, myoblast

Cellular systems studied: cell lines, primary cells, tissue

Species studied: human, mouse, rat

Upstream Regulation

Treatments, proteins and their effect on site modification:

Treatments	Referenced Treatments	Manipulated Protein	Referenced Protein	Effect	Notes
IL-6				no change compared to control	Muscle
IL-6				increase	Liver

S318-p - IRS1 (mouse)

▲

Orthologous residues

IRS1 (human): S323‑p, IRS1 (mouse): S318‑p, IRS1 (rat): S318‑p

Characterization

Methods used to characterize site in vivo: mutation of modification site, phospho-antibody, western blotting

Relevant cell lines - cell types - tissues: 'muscle, skeletal', C2C12 (myoblast), L6 (myoblast), liver, myoblast

Cellular systems studied: cell lines, primary cells, tissue

Species studied: human, mouse, rat

Upstream Regulation

Potential in vivo enzymes for site:

Type	Enzyme	Evidence	Notes
KINASE	PKCD (mouse)	transfection of wild-type enzyme, co-immunoprecipitation, siRNA inhibition of enzyme, phospho-antibody, transfection of inactive enzyme

Treatments, proteins and their effect on site modification:

Treatments	Manipulated Protein	Effect	Notes
IL-6		increase	Muscle, C2C12, L6, 1' human myotubes
IL-6		no change compared to control	Liver
	PKCD (human)	increase	C2C12 cells
siRNA		decrease	PKC delta siRNA

Downstream Regulation

Effect of modification (function): phosphorylation

Comments: Phosphorylation of S318 leads to an increase of phospho-Ser473 Akt1 in L6GLUT4 myotubes.

Y705-p - STAT3 (mouse)

▲

Orthologous residues

STAT3 (human): Y705‑p, STAT3 iso2 (human): Y704‑p, STAT3 iso3 (human): Y705‑p, STAT3 (mouse): Y705‑p, STAT3 iso2 (mouse): Y705‑p, STAT3 iso3 (mouse): Y704‑p, STAT3 (rat): Y705‑p, STAT3 (cow): Y705‑p

Characterization

Methods used to characterize site in vivo: phospho-antibody, western blotting

Relevant cell lines - cell types - tissues: 'muscle, skeletal', C2C12 (myoblast), L6 (myoblast), liver, myoblast

Cellular systems studied: cell lines, primary cells, tissue

Species studied: human, mouse, rat

Upstream Regulation

Treatments, proteins and their effect on site modification:

Treatments	Referenced Treatments	Manipulated Protein	Referenced Protein	Effect	Notes
IL-6				increase	Liver, muscle, C2C12, L6, 1' human myotubes

Home \| Curator Login	With enhanced literature mining using Linguamatics I2E		Produced by 3rd Millennium \| Design by Digizyme
			©2003-2013 Cell Signaling Technology, Inc.