Curated Information
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Curated Information Page
PubMed Id: 21474068 
Chang CC, et al. (2011) Structural and functional roles of Daxx SIM phosphorylation in SUMO paralog-selective binding and apoptosis modulation. Mol Cell 42, 62-74 21474068
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
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S178-p - DAXX (human)
Orthologous residues
DAXX (human): S178‑p, DAXX (mouse): A184‑p, DAXX (rat): A176‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

S213-p - DAXX (human)
Orthologous residues
DAXX (human): S213‑p, DAXX (mouse): S219‑p, DAXX (rat): S211‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

S246-p - DAXX (human)
Orthologous residues
DAXX (human): S246‑p, DAXX (mouse): S252‑p, DAXX (rat): S244‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

T249-p - DAXX (human)
Orthologous residues
DAXX (human): T249‑p, DAXX (mouse): T255‑p, DAXX (rat): T247‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

S495-p - DAXX (human)
Orthologous residues
DAXX (human): S495‑p, DAXX (mouse): S502‑p, DAXX (rat): S485‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

S498-p - DAXX (human)
Orthologous residues
DAXX (human): S498‑p, DAXX (mouse): S505‑p, DAXX (rat): S488‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

T665-p - DAXX (human)
Orthologous residues
DAXX (human): T665‑p, DAXX (mouse): V664‑p, DAXX (rat): V656‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

S671-p - DAXX (human)
Orthologous residues
DAXX (human): S671‑p, DAXX (mouse): P670‑p, DAXX (rat): S662‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

S688-p - DAXX (human)
Orthologous residues
DAXX (human): S688‑p, DAXX (mouse): S687‑p, DAXX (rat): S679‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

S707-p - DAXX (human)
Orthologous residues
DAXX (human): S707‑p, DAXX (mouse): L706‑p, DAXX (rat): L698‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

T709-p - DAXX (human)
Orthologous residues
DAXX (human): T709‑p, DAXX (mouse): T708‑p, DAXX (rat): T700‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

S737-p - DAXX (human)
Orthologous residues
DAXX (human): S737‑p, DAXX (mouse): S736‑p, DAXX (rat): S728‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK2A1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sorbitol increase
siRNA sorbitol inhibit treatment-induced increase shRNA CK2
TBB sorbitol inhibit treatment-induced increase
DMAT sorbitol inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, sumoylation
 Effect of modification (process):  apoptosis, altered, transcription, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PML (human) Induces co-immunoprecipitation
SUMO1 (human) Induces isothermal titration calorimetry (ITC)

S739-p - DAXX (human)
Orthologous residues
DAXX (human): S739‑p, DAXX (mouse): S738‑p, DAXX (rat): S730‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK2A1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sorbitol increase
siRNA sorbitol inhibit treatment-induced increase shRNA CK2
TBB sorbitol inhibit treatment-induced increase
DMAT sorbitol inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, sumoylation
 Effect of modification (process):  apoptosis, altered, transcription, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PML (human) Induces co-immunoprecipitation
SUMO1 (human) Induces isothermal titration calorimetry (ITC)

K39-sm - SUMO1 (human)
Orthologous residues
SUMO1 (human): K39‑sm, SUMO1 iso2 (human): K14‑sm, SUMO1 (mouse): K39‑sm, SUMO1 (rat): K39‑sm
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
DAXX (human) Induces isothermal titration calorimetry (ITC)


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