Curated Information
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Curated Information Page
PubMed Id: 21474068 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Chang CC, et al. (2011) Structural and functional roles of Daxx SIM phosphorylation in SUMO paralog-selective binding and apoptosis modulation. Mol Cell 42, 62-74 21474068
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S178-p - Daxx (human)
Orthologous residues
Daxx (human): S178‑p, Daxx (mouse): A184‑p, Daxx (rat): A176‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

S213-p - Daxx (human)
Orthologous residues
Daxx (human): S213‑p, Daxx (mouse): S219‑p, Daxx (rat): S211‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

S246-p - Daxx (human)
Orthologous residues
Daxx (human): S246‑p, Daxx (mouse): S252‑p, Daxx (rat): S244‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

T249-p - Daxx (human)
Orthologous residues
Daxx (human): T249‑p, Daxx (mouse): T255‑p, Daxx (rat): T247‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

S495-p - Daxx (human)
Orthologous residues
Daxx (human): S495‑p, Daxx (mouse): S502‑p, Daxx (rat): S485‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

S498-p - Daxx (human)
Orthologous residues
Daxx (human): S498‑p, Daxx (mouse): S505‑p, Daxx (rat): S488‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

T665-p - Daxx (human)
Orthologous residues
Daxx (human): T665‑p, Daxx (mouse): , Daxx (rat):
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

S671-p - Daxx (human)
Orthologous residues
Daxx (human): S671‑p, Daxx (mouse): P670‑p, Daxx (rat): S662‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

S688-p - Daxx (human)
Orthologous residues
Daxx (human): S688‑p, Daxx (mouse): S687‑p, Daxx (rat): S679‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

S707-p - Daxx (human)
Orthologous residues
Daxx (human): S707‑p, Daxx (mouse): , Daxx (rat):
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

T709-p - Daxx (human)
Orthologous residues
Daxx (human): T709‑p, Daxx (mouse): T708‑p, Daxx (rat): T700‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human

S737-p - Daxx (human)
Orthologous residues
Daxx (human): S737‑p, Daxx (mouse): S736‑p, Daxx (rat): S728‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK2A1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sorbitol increase
siRNA sorbitol inhibit treatment-induced increase shRNA CK2
TBB sorbitol inhibit treatment-induced increase
DMAT sorbitol inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, sumoylation
 Effect of modification (process):  apoptosis, altered, transcription, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SUMO1 (human) Induces isothermal titration calorimetry (ITC)
PML (human) Induces co-immunoprecipitation

S739-p - Daxx (human)
Orthologous residues
Daxx (human): S739‑p, Daxx (mouse): S738‑p, Daxx (rat): S730‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK2A1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sorbitol increase
siRNA sorbitol inhibit treatment-induced increase shRNA CK2
TBB sorbitol inhibit treatment-induced increase
DMAT sorbitol inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, sumoylation
 Effect of modification (process):  apoptosis, altered, transcription, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PML (human) Induces co-immunoprecipitation
SUMO1 (human) Induces isothermal titration calorimetry (ITC)

K39-s - SUMO1 (human)
Orthologous residues
SUMO1 (human): K39‑s, SUMO1 iso2 (human): K14‑s, SUMO1 (mouse): K39‑s, SUMO1 (rat): K39‑s
Characterization
 Methods used to characterize site in vivo mutation of modification site
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Daxx (human) Induces isothermal titration calorimetry (ITC)


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