Curated Information
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Curated Information Page
PubMed Id: 16291744 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Zhang Z, Lin SY, Neel BG, Haimovich B (2006) Phosphorylated alpha-actinin and protein-tyrosine phosphatase 1B coregulate the disassembly of the focal adhesion kinase x Src complex and promote cell migration. J Biol Chem 281, 1746-54 16291744
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y12-p - ACTN1 (human) ▲
Orthologous residues
ACTN1 (human): Y12‑p, ACTN1 (mouse): Y12‑p, ACTN1 iso3 (mouse): Y12‑p, ACTN1 (rat): Y12‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site
 Relevant cell lines - cell types - tissues:  COS (fibroblast), MEF (fibroblast) [PTP1B (mouse)]
 Cellular systems studied:  cell lines
 Species studied:  monkey, mouse
 Comments:  MEF PTP 1B -/-
Downstream Regulation
 Effect of modification (function):  molecular association, regulation, phosphorylation
 Effect of modification (process):  cell motility, altered
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Src (human) Induces molecular association, regulation pull-down assay, co-immunoprecipitation
 Comments:  Phosphorylation of ACTN1 Y12 is required for PTP 1B to dephosphorylate FAK Y397. Y12F mutation or no expression of ACTN1 significantly decreases the FAK Y397 dephosphorylation by PTP 1B. Src's binding to phospho-Y12 ACTN1 competes with Src's binding to FAK.

Y397-p - FAK (human) ▲
Orthologous residues
FAK (human): Y397‑p, FAK iso2 (human): Y216‑p, FAK iso5 (human): Y397‑p, FAK (mouse): Y428‑p, FAK iso3 (mouse): Y397‑p, FAK iso4 (mouse): Y397‑p, FAK (rat): Y397‑p, FAK (chicken): Y397‑p, FAK iso5 (chicken): ‑
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), MEF (fibroblast) [PTP1B (mouse)]
 Cellular systems studied:  cell lines
 Species studied:  monkey, mouse
 Comments:  MEF PTP1B -/-
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PTP1B (mouse) genetic knockout/knockin of upstream enzyme, transfection of inactive enzyme, transfection of wild-type enzyme, phospho-antibody

Y407-p - FAK (human) ▲
Orthologous residues
FAK (human): Y407‑p, FAK iso2 (human): Y226‑p, FAK iso5 (human): Y407‑p, FAK (mouse): Y438‑p, FAK iso3 (mouse): Y407‑p, FAK iso4 (mouse): Y407‑p, FAK (rat): Y407‑p, FAK (chicken): Y407‑p, FAK iso5 (chicken): ‑
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey

Y576-p - FAK (human) ▲
Orthologous residues
FAK (human): Y576‑p, FAK iso2 (human): Y424‑p, FAK iso5 (human): Y576‑p, FAK (mouse): Y614‑p, FAK iso3 (mouse): Y576‑p, FAK iso4 (mouse): Y576‑p, FAK (rat): Y576‑p, FAK (chicken): Y576‑p, FAK iso5 (chicken): ‑
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey

Y577-p - FAK (human) ▲
Orthologous residues
FAK (human): Y577‑p, FAK iso2 (human): Y425‑p, FAK iso5 (human): Y577‑p, FAK (mouse): Y615‑p, FAK iso3 (mouse): Y577‑p, FAK iso4 (mouse): Y577‑p, FAK (rat): Y577‑p, FAK (chicken): Y577‑p, FAK iso5 (chicken): ‑
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey

Y861-p - FAK (human) ▲
Orthologous residues
FAK (human): Y861‑p, FAK iso2 (human): Y688‑p, FAK iso5 (human): Y861‑p, FAK (mouse): Y899‑p, FAK iso3 (mouse): Y861‑p, FAK iso4 (mouse): Y861‑p, FAK (rat): Y861‑p, FAK (chicken): Y863‑p, FAK iso5 (chicken): Y169‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  monkey

Y419-p - Src (human) ▲
Orthologous residues
Src (human): Y419‑p, Src (mouse): Y424‑p, Src iso2 (mouse): Y418‑p, Src (rat): Y419‑p, Src (chicken): Y416‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast) [PTP1B (mouse)]
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Comments:  MEF PTP1B -/-

Y530-p - Src (human) ▲
Orthologous residues
Src (human): Y530‑p, Src (mouse): Y535‑p, Src iso2 (mouse): Y529‑p, Src (rat): Y530‑p, Src (chicken): Y527‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  MEF (fibroblast) [PTP1B (mouse)]
 Cellular systems studied:  cell lines
 Species studied:  mouse
 Comments:  MEF PTP1B -/-


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