Curated Information
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PubMed Id: 16219803 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Pandey D, Goyal P, Bamburg JR, Siess W (2006) Regulation of LIM-kinase 1 and cofilin in thrombin-stimulated platelets. Blood 107, 575-83 16219803
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S3-p - Cofilin-1 (human)
Orthologous residues
Cofilin‑1 (human): S3‑p, Cofilin‑1 (mouse): S3‑p, Cofilin‑1 (rat): S3‑p, Cofilin‑1 (pig): S3‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  platelet-blood
 Cellular systems studied:  primary cells
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
thrombin decrease Rapidly decreases and returns to resting level within 2 minutes.
Y27632 thrombin augment treatment-induced decrease
Y27632 decrease
H1152 thrombin augment treatment-induced decrease
RGDS thrombin no change compared to control

T508-p - LIMK1 (human)
Orthologous residues
LIMK1 (human): T508‑p, LIMK1 (mouse): T508‑p, LIMK1 (rat): T508‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  platelet-blood
 Cellular systems studied:  primary cells
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
thrombin increase
H1152 thrombin inhibit treatment-induced increase
Y27632 thrombin inhibit treatment-induced increase
RGDS thrombin augment treatment-induced increase Authors claim no change in the phosphorylation kinetics pattern in +RGDS (blot appears to show a noticeable increase)

T696-p - MYPT1 (human)
Orthologous residues
MYPT1 (human): T696‑p, MYPT1 iso4 (human): T637‑p, MYPT1 (mouse): T694‑p, MYPT1 (rat): T697‑p, MYPT1 (pig): T696‑p, MYPT1 (chicken): T695‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  platelet-blood
 Cellular systems studied:  primary cells
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
thrombin increase
Y27632 thrombin inhibit treatment-induced increase
H1152 thrombin inhibit treatment-induced increase
RGDS thrombin augment treatment-induced increase

T853-p - MYPT1 (human)
Orthologous residues
MYPT1 (human): T853‑p, MYPT1 iso4 (human): T794‑p, MYPT1 (mouse): T852‑p, MYPT1 (rat): T855‑p, MYPT1 (pig): , MYPT1 (chicken): T850‑p
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  platelet-blood
 Cellular systems studied:  primary cells
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
thrombin increase
Y27632 thrombin inhibit treatment-induced increase
H1152 thrombin inhibit treatment-induced increase
RGDS thrombin augment treatment-induced increase


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