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Orthologous residues
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Titin (human): Y32341‑p, Titin iso3 (human): Y24917‑p, Titin iso6 (human): ‑, Titin (mouse): Y33203‑p, Titin iso3 (mouse): ‑, Titin iso4 (mouse): Y31457‑p, Titin (rat): Y33089‑p, Titin iso2 (rat): ‑
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Characterization
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Methods used to characterize site in vivo:
mutation of modification site, phospho-antibody
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Relevant cell lines - cell types - tissues:
C2C12 (myoblast)
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Comments:
The large size of titin (2993428 Da /26,926 a.a.) precluded transfecting whole gene. For these studies, only the kinase domain was studied. Tyr 24917 is refered to as tyr 170 in this paper. Like ERK2, Tyr 24917 is buried in the inhibited, unphosphorylated structure of titin kinase.
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Downstream Regulation
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Effect of modification (function):
enzymatic activity, induced
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