Curated Information
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Curated Information Page
PubMed Id: 11546811 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Block K, Boyer TG, Yew PR (2001) Phosphorylation of the human ubiquitin-conjugating enzyme, CDC34, by casein kinase 2. J Biol Chem 276, 41049-58 11546811
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S203-p - Cdc34 (human) ▲
Orthologous residues
Cdc34 (human): S203‑p, Cdc34 (mouse): S203‑p, Cdc34 (rat): S203‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  C3H10T1/2 (fibroblast) [Cdc34 (human)], U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE CK2A1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) co-immunoprecipitation
Downstream Regulation
 Effect of modification (function):  intracellular localization

S222-p - Cdc34 (human) ▲
Orthologous residues
Cdc34 (human): S222‑p, Cdc34 (mouse): S221‑p, Cdc34 (rat): S221‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  C3H10T1/2 (fibroblast) [Cdc34 (human)], U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE CK2A1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) co-immunoprecipitation
Downstream Regulation
 Effect of modification (function):  intracellular localization

S231-p - Cdc34 (human) ▲
Orthologous residues
Cdc34 (human): S231‑p, Cdc34 (mouse): S230‑p, Cdc34 (rat): S230‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  C3H10T1/2 (fibroblast) [Cdc34 (human)], U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE CK2A1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) co-immunoprecipitation
Downstream Regulation
 Effect of modification (function):  intracellular localization

T233-p - Cdc34 (human) ▲
Orthologous residues
Cdc34 (human): T233‑p, Cdc34 (mouse): T232‑p, Cdc34 (rat): T232‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  C3H10T1/2 (fibroblast) [Cdc34 (human)], U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE CK2A1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) co-immunoprecipitation
Downstream Regulation
 Effect of modification (function):  intracellular localization

S236-p - Cdc34 (human) ▲
Orthologous residues
Cdc34 (human): S236‑p, Cdc34 (mouse): S235‑p, Cdc34 (rat): S235‑p
Characterization
 Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site
 Disease tissue studied:  bone cancer
 Relevant cell lines - cell types - tissues:  C3H10T1/2 (fibroblast) [Cdc34 (human)], U2OS (bone cell)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE CK2A1 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) co-immunoprecipitation
Downstream Regulation
 Effect of modification (function):  intracellular localization


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