Curated Information
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Curated Information Page
PubMed Id: 21293379 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Pei H, et al. (2011) MMSET regulates histone H4K20 methylation and 53BP1 accumulation at DNA damage sites. Nature 470, 124-8 21293379
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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K37-m3 - H3 (human)
Orthologous residues
H3 (human): K37‑m3, H3 (mouse): K37‑m3, H3 iso2 (mouse): K37‑m3, H3 iso3 (mouse): K37‑m3, H3 (rat): K37‑m3, H3 iso3 (rat): K37‑m3, H3 (pig): K37‑m3, H3 (chicken): K37‑m3, H3 (cow): K37‑m3
Characterization
 Methods used to characterize site in vivo phospho-antibody, western blotting
 Disease tissue studied:  colorectal cancer, colorectal carcinoma
 Relevant cell lines - cell types - tissues:  HCT116 (intestinal), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DSBs no change compared to control
siRNA no effect upon treatment-induced increase shRNA WHSC1 (aka MMSET)

K21-m1 - H4 (human)
Orthologous residues
H4 (human): K21‑m1, H4 (mouse): K21‑m1, H4 (rat): K21‑m1, H4 (pig): K21‑m1, H4 (cow): K21‑m1
Characterization
 Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DSBs increase DSBs induced by overexpression of I-SceI (intron-encoded endonuclease from yeast)

K21-m2 - H4 (human)
Orthologous residues
H4 (human): K21‑m2, H4 (mouse): K21‑m2, H4 (rat): K21‑m2, H4 (pig): K21‑m2, H4 (cow): K21‑m2
Characterization
 Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
METHYLTRANSFERASE WHSC1 (human) activation of upstream enzyme, transfection of inactive enzyme, siRNA inhibition of enzyme, pharmacological activator of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DSBs increase DSBs induced by overexpression of I-SceI (intron-encoded endonuclease from yeast)
siRNA DSBs inhibit treatment-induced increase shRNA WHSC1 (aka MMSET)

K21-m3 - H4 (human)
Orthologous residues
H4 (human): K21‑m3, H4 (mouse): K21‑m3, H4 (rat): K21‑m3, H4 (pig): K21‑m3, H4 (cow): K21‑m3
Characterization
 Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
METHYLTRANSFERASE WHSC1 (human) activation of upstream enzyme, transfection of inactive enzyme, siRNA inhibition of enzyme, pharmacological activator of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DSBs increase DSBs induced by overexpression of I-SceI (intron-encoded endonuclease from yeast)
siRNA DSBs inhibit treatment-induced increase shRNA WHSC1 (aka MMSET)

S102-p - WHSC1 (human)
Orthologous residues
WHSC1 (human): S102‑p, WHSC1 iso3 (human): S102‑p, WHSC1 iso5 (human): S102‑p, WHSC1 (mouse): A102‑p, WHSC1 iso3 (mouse): A102‑p, WHSC1 (rat): T102‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  colorectal cancer, colorectal carcinoma
 Relevant cell lines - cell types - tissues:  293T (epithelial), HCT116 (intestinal), HeLa (cervical)
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) genetic knockout/knockin of upstream enzyme, modification site within consensus motif
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing radiation increase
Downstream Regulation
 Effect of modification (function):  intracellular localization, methylation, molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
MDC1 (human) Induces co-immunoprecipitation
 Comments:  essential for WHSC1 accumulation at DNA damage sites; important for H4K20 methylation;


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