Curated Information
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PubMed Id: 21282370 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Aboulaich N, et al. (2011) Polymerase I and Transcript Release Factor Regulates Lipolysis via a Phosphorylation-Dependent Mechanism. Diabetes 60, 757-65 21282370
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S870-p - HSL (mouse) ▲
Orthologous residues
HSL (human): S853‑p, HSL iso2 (human): S552‑p, HSL (mouse): S870‑p, HSL (rat): S863‑p, HSL iso2 (rat): S563‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PTRF (mouse) increase

S964-p - HSL (mouse) ▲
Orthologous residues
HSL (human): S951‑p, HSL iso2 (human): S650‑p, HSL (mouse): S964‑p, HSL (rat): S960‑p, HSL iso2 (rat): S660‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
 Cellular systems studied:  cell lines
 Species studied:  mouse
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PTRF (mouse) increase

S21-p - PTRF (mouse) ▲
Orthologous residues
PTRF (human): ‑, PTRF (mouse): S21‑p, PTRF iso3 (mouse): S21‑p, PTRF (rat): S21‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site
 Relevant cell lines - cell types - tissues:  adipose tissue
 Cellular systems studied:  tissue
 Species studied:  mouse

S42-p - PTRF (mouse) ▲
Orthologous residues
PTRF (human): S40‑p, PTRF (mouse): S42‑p, PTRF iso3 (mouse): S42‑p, PTRF (rat): S42‑p
Characterization
 Methods used to characterize site in vivo:  mutation of modification site
 Relevant cell lines - cell types - tissues:  adipose tissue
 Cellular systems studied:  tissue
 Species studied:  mouse
Downstream Regulation
 Effect of modification (function):  phosphorylation
 Comments:  regulates lipolysis in adipocytes; regulates phosphorylation of HSL S557 (aka S563) and S651 (aka S660)

S169-p - PTRF (mouse) ▲
Orthologous residues
PTRF (human): S167‑p, PTRF (mouse): S169‑p, PTRF iso3 (mouse): ‑, PTRF (rat): S169‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry
 Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast), adipose tissue
 Cellular systems studied:  cell lines, tissue
 Species studied:  mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKACa (mouse) modification site within consensus motif
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fasting increase

S171-p - PTRF (mouse) ▲
Orthologous residues
PTRF (human): S169‑p, PTRF (mouse): S171‑p, PTRF iso3 (mouse): ‑, PTRF (rat): S171‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry
 Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast), adipose tissue
 Cellular systems studied:  cell lines, tissue
 Species studied:  mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKACa (mouse) modification site within consensus motif
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fasting increase

T304-p - PTRF (mouse) ▲
Orthologous residues
PTRF (human): T302‑p, PTRF (mouse): T304‑p, PTRF iso3 (mouse): ‑, PTRF (rat): T304‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast), adipose tissue
 Cellular systems studied:  cell lines, tissue
 Species studied:  mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKACa (mouse) modification site within consensus motif
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fasting increase
Downstream Regulation
 Effect of modification (function):  phosphorylation
 Comments:  regulates lipolysis in adipocytes; regulates phosphorylation of HSL S557 (aka S563) and S651 (aka S660)

S368-p - PTRF (mouse) ▲
Orthologous residues
PTRF (human): S366‑p, PTRF (mouse): S368‑p, PTRF iso3 (mouse): ‑, PTRF (rat): S368‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast), adipose tissue
 Cellular systems studied:  cell lines, tissue
 Species studied:  mouse
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKACa (mouse) modification site within consensus motif
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fasting increase
Downstream Regulation
 Effect of modification (function):  phosphorylation
 Comments:  regulates lipolysis in adipocytes; regulates phosphorylation of HSL S557 (aka S563) and S651 (aka S660)


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