Curated Information
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Curated Information Page
PubMed Id: 21228273 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Vigetti D, et al. (2011) Hyaluronan Synthesis Is Inhibited by Adenosine Monophosphate-activated Protein Kinase through the Regulation of HAS2 Activity in Human Aortic Smooth Muscle Cells. J Biol Chem 286, 7917-24 21228273
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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T172-p - AMPKA2 (human) ▲
Orthologous residues
AMPKA2 (human): T172‑p, AMPKA2 (mouse): T172‑p, AMPKA2 (rat): T172‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'muscle, smooth'-thoracic aorta
 Cellular systems studied:  primary cultured cells
 Species studied:  human
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
AICAR increase
compound C AICAR inhibit treatment-induced increase
metformin increase
compound C metformin inhibit treatment-induced increase
2-deoxyglucose increase

T110-p - HAS2 (human) ▲
Orthologous residues
HAS2 (human): T110‑p, HAS2 (mouse): T110‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  COS (fibroblast), COS (fibroblast) [AMPKA2 (human), transfection]
 Cellular systems studied:  cell lines
 Species studied:  monkey
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE AMPKA2 (human) transfection of constitutively active enzyme, transfection of dominant-negative enzyme, transfection of wild-type enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
AICAR increase
compound C AICAR inhibit treatment-induced increase
metformin increase
compound C metformin inhibit treatment-induced increase
2-deoxyglucose increase
Downstream Regulation
 Effect of modification (function):  enzymatic activity, inhibited
 Effect of modification (process):  cell adhesion, altered, cell motility, altered


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