Curated Information
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Curated Information Page
PubMed Id: 21264250 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Francis J, Lin W, Rozenblatt-Rosen O, Meyerson M (2011) The menin tumor suppressor protein is phosphorylated in response to DNA damage. PLoS One 6, e16119 21264250
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S394-p - MEN1 iso2 (human)
Orthologous residues
MEN1 (human): S399‑p, MEN1 iso2 (human): S394‑p, MEN1 (mouse): A394‑p, MEN1 (rat): A394‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Disease tissue studied:  ataxia-telangiectasia
 Relevant cell lines - cell types - tissues:  293T (epithelial), GM03189 (lymphoblastoid), GM03323, GM18326
 Cellular systems studied:  cell lines
 Species studied:  human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) pharmacological activator of upstream enzyme, pharmacological inhibitor of upstream enzyme, genetic knockout/knockin of upstream enzyme
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
KU-55933 UV no effect upon treatment-induced increase
DNA-PK inhibitor II UV no effect upon treatment-induced increase
ionizing radiation increase
KU-55933 ionizing radiation inhibit treatment-induced increase
DNA-PK inhibitor II ionizing radiation no effect upon treatment-induced increase
adriamycin increase
Downstream Regulation
 Effect of modification (function):  phosphorylation
 Comments:  important for dephosphorylation of S487

S487-p - MEN1 iso2 (human)
Orthologous residues
MEN1 (human): S492‑p, MEN1 iso2 (human): S487‑p, MEN1 (mouse): S487‑p, MEN1 (rat): S487‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S543-p - MEN1 iso2 (human)
Orthologous residues
MEN1 (human): S548‑p, MEN1 iso2 (human): S543‑p, MEN1 (mouse): S544‑p, MEN1 (rat): S543‑p
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293T (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  molecular association, regulation
 Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
POLR2A (human) Disrupts co-immunoprecipitation

S487-p - MEN1 (mouse)
Orthologous residues
MEN1 (human): S492‑p, MEN1 iso2 (human): S487‑p, MEN1 (mouse): S487‑p, MEN1 (rat): S487‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Disease tissue studied:  pancreatic cancer, pancreatic carcinoma
 Relevant cell lines - cell types - tissues:  beta TC3 (pancreatic)
 Cellular systems studied:  cell lines
 Species studied:  mouse

S544-p - MEN1 (mouse)
Orthologous residues
MEN1 (human): S548‑p, MEN1 iso2 (human): S543‑p, MEN1 (mouse): S544‑p, MEN1 (rat): S543‑p
Characterization
 Methods used to characterize site in vivo mass spectrometry
 Disease tissue studied:  pancreatic cancer, pancreatic carcinoma
 Relevant cell lines - cell types - tissues:  beta TC3 (pancreatic)
 Cellular systems studied:  cell lines
 Species studied:  mouse


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