Curated Information
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Curated Information Page
PubMed Id: 21209322 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Gallazzini M, et al. (2011) High NaCl-induced activation of CDK5 increases phosphorylation of the osmoprotective transcription factor TonEBP/OREBP at threonine 135, which contributes to its rapid nuclear localization. Mol Biol Cell 22, 703-14 21209322
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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S120-p - NFAT5 (human) ▲
Orthologous residues
NFAT5 (human): S120‑p, NFAT5 (mouse): S120‑p, NFAT5 iso2 (mouse): S138‑p, NFAT5 (rat): S137‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human

S134-p - NFAT5 (human) ▲
Orthologous residues
NFAT5 (human): S134‑p, NFAT5 (mouse): S134‑p, NFAT5 iso2 (mouse): S152‑p, NFAT5 (rat): S151‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  intracellular localization

T135-p - NFAT5 (human) ▲
Orthologous residues
NFAT5 (human): T135‑p, NFAT5 (mouse): T135‑p, NFAT5 iso2 (mouse): T153‑p, NFAT5 (rat): T152‑p
Characterization
 Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
 Enzymes shown to modify site in vitro: 
Type Enzyme
KINASE CDK5 (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CDK5 (human) pharmacological inhibitor of upstream enzyme, pharmacological activator of upstream enzyme, modification site within consensus motif, co-immunoprecipitation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
NaCl increase
Cdk1/5 inhibitor NaCl inhibit treatment-induced increase
roscovitine NaCl inhibit treatment-induced increase
Downstream Regulation
 Effect of modification (function):  intracellular localization
 Effect of modification (process):  transcription, altered

S155-p - NFAT5 (human) ▲
Orthologous residues
NFAT5 (human): S155‑p, NFAT5 (mouse): S155‑p, NFAT5 iso2 (mouse): S173‑p, NFAT5 (rat): S172‑p
Characterization
 Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site
 Relevant cell lines - cell types - tissues:  293 (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  human
Downstream Regulation
 Effect of modification (function):  intracellular localization

T152-p - NFAT5 (rat) ▲
Orthologous residues
NFAT5 (human): T135‑p, NFAT5 (mouse): T135‑p, NFAT5 iso2 (mouse): T153‑p, NFAT5 (rat): T152‑p
Characterization
 Methods used to characterize site in vivo:  phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  'kidney, inner medulla'
 Cellular systems studied:  tissue
 Species studied:  rat
Upstream Regulation
 Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
dDAVP increase


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