Curated Information
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Curated Information Page
PubMed Id: 20802513 
This page summarizes selected information from the article referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2012,40:D261-70). To learn more about the scope of PhosphoSitePlus®, click here.
Chen TH, Chan PC, Chen CL, Chen HC (2011) Phosphorylation of focal adhesion kinase on tyrosine 194 by Met leads to its activation through relief of autoinhibition. Oncogene 30, 153-66 20802513
Only sites from this record are displayed on this page. Click on the protein name to open the protein page, and on the RSD number to open the site page. For the complete dataset, click the download button, on the right.
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Y5-p - FAK (human)
Orthologous residues
FAK (human): Y5‑p, FAK iso2 (human): , FAK iso5 (human): Y5‑p, FAK (mouse): Y5‑p, FAK iso3 (mouse): Y5‑p, FAK iso4 (mouse): Y5‑p, FAK (rat): Y5‑p, FAK (chicken): Y5‑p, FAK iso5 (chicken):
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), MDCK (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  dog, human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE Met (human)
KINASE PDGFRB (human)
KINASE EGFR (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PDGFRB (human) transfection of wild-type enzyme
KINASE EGFR (human) transfection of wild-type enzyme
KINASE Met (human) transfection of wild-type enzyme

Y194-p - FAK (human)
Orthologous residues
FAK (human): Y194‑p, FAK iso2 (human): Y13‑p, FAK iso5 (human): Y194‑p, FAK (mouse): Y194‑p, FAK iso3 (mouse): Y194‑p, FAK iso4 (mouse): Y194‑p, FAK (rat): Y194‑p, FAK (chicken): Y194‑p, FAK iso5 (chicken):
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), MDCK (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  dog, human
 Enzymes shown to modify site in vitro
Type Enzyme
KINASE EGFR (human)
KINASE PDGFRB (human)
KINASE Met (human)
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE EGFR (human) transfection of wild-type enzyme
KINASE Met (human) transfection of wild-type enzyme
KINASE PDGFRB (human) transfection of wild-type enzyme
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced, phosphorylation, protein conformation
 Effect of modification (process):  cell growth, altered, cell motility, altered
 Comments:  activates FAK and allows it to phosphorylate itself on Y397

Y397-p - FAK (human)
Orthologous residues
FAK (human): Y397‑p, FAK iso2 (human): Y216‑p, FAK iso5 (human): Y397‑p, FAK (mouse): Y428‑p, FAK iso3 (mouse): Y397‑p, FAK iso4 (mouse): Y397‑p, FAK (rat): Y397‑p, FAK (chicken): Y397‑p, FAK iso5 (chicken):
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), MDCK (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  dog, human
Upstream Regulation
 Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE FAK (human) activation of upstream enzyme
Downstream Regulation
 Effect of modification (function):  enzymatic activity, induced
 Effect of modification (process):  cell growth, altered, cell motility, altered

Y577-p - FAK (human)
Orthologous residues
FAK (human): Y577‑p, FAK iso2 (human): Y425‑p, FAK iso5 (human): Y577‑p, FAK (mouse): Y615‑p, FAK iso3 (mouse): Y577‑p, FAK iso4 (mouse): Y577‑p, FAK (rat): Y577‑p, FAK (chicken): Y577‑p, FAK iso5 (chicken):
Characterization
 Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
 Relevant cell lines - cell types - tissues:  293 (epithelial), MDCK (epithelial)
 Cellular systems studied:  cell lines
 Species studied:  dog, human


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